Education PhD Department of Biological Sciences, National University of Singapore (2007) MSc Institute of Genetics and Cytology, Northeast Normal University, Changchun, China (2002) BSc School of Life Science, Northeast Normal University, Changchun, China (1999) Academic and Professional Experience 2015-present Assistant Professor, Faculty of Health Sciences, University of Macau 2013.4-2014.12 Senior Scientist /Team Leader, Vela Diagnostics, Singapore 2012.1-2013.3 Scientist, Vela Diagnostics, Singapore 2009.9-2011.12 Research Fellow, Novartis Institute for Tropical Diseases, Singapore 2008.3-2009.8 Research Fellow, Dept. of Microbiology and Molecular Genetics, Harvard Medical School, USA, Advisor: Dr. John Mekalanos Course Outlines HSCI2002 General and Clinical Microbiology HSCI3002 Immunology and Infectious Diseases

Mechanism of bacterial death Bacterial pathogenesis Development of new antibacterials

Representative Publications Zou, J., Kou, S. H., Xie, R. Q., VanNieuwenhze, M. S., Qu, J. X., Peng, B., and Zheng, J. (2020) Non-Walled Spherical Acinetobacter Baumannii Is an Important Type of Persisters Upon Beta-Lactam Antibiotics Treatment. Emerg Microbes Infect 9 (1), 1149-1159 Zheng, J. (2020) SARS-CoV-2: An Emerging Coronavirus That Causes a Global Threat. Int J Biol Sci 16 (10), 1678-1685 Zhang, W. W., Xie, R. Q., Zhang, X. H. D., Lee, L., Zhang, H. J., Yang, M. H., Peng, B., and Zheng, J. (2020) Organism Dual RNA-Seq Reveals the Importance of BarA/UvrY in Vibrio Parahaemolyticus Virulence. Faseb J Jiang, M., Yang, L. F., Chen, Z. G., Lai, S. S., Zheng, J., and Peng, B. (2020) Exogenous Maltose Enhances Zebrafish Immunity to Levofloxacin-Resistant Vibrio Alginolyticus. Microb Biotechnol 13 (4), 1213-1227 Ji, X., Zou, J., Peng, H. B., Stolle, A.-S., Xie, R. Q., Zhang, H. J., Peng, B., Mekalanos, J. J., and Zheng, J. (2019) Alarmone Ap4A Is Elevated by Aminoglycoside Antibiotics and Enhances Their Bactericidal Activity. P Natl Acad Sci Usa 116 (19), 9578-9585 Zou, J., Zhang, W. W., Zhang, H. J., Zhang, X. H. D., Peng, B., and Zheng, J. (2018) Studies on Aminoglycoside Susceptibility Identify a Novel Function of KsgA to Secure Translational Fidelity During Antibiotic Stress. Antimicrob Agents Ch 62 (10), e00853-00818 Zhang, Y. C., Faucher, F., Zhang, W. W., Wang, S., Neville, N., Poole, K., Zheng, J., and Jia, Z. C. (2018) Structure-Guided Disruption of the Pseudopilus Tip Complex Inhibits the Type II Secretion in Pseudomonas Aeruginosa. Plos Pathog 14 (10), e1007343 Ye, J.-Z., Su, Y.-B., Lin, X.-M., Lai, S.-S., Li, W.-X., Ali, F., Zheng, J., and Peng, B. (2018) Alanine Enhances Aminoglycosides-Induced ROS Production as Revealed by Proteomic Analysis. Front Microbiol 9, 29 Ye, J. Z., Lin, X. M., Cheng, Z. X., Su, Y. B., Li, W. X., Ali, F. M., Zheng, J., and Peng, B. (2018) Identification and Efficacy of Glycine, Serine and Threonine Metabolism in Potentiating Kanamycin-Mediated Killing of Edwardsiella Piscicida. J Proteomics 183, 34-44 Xie, R. Q., Zhang, X. H. D., Zhao, Q., Peng, B., and Zheng, J. (2018) Analysis of Global Prevalence of Antibiotic Resistance in Acinetobacter Baumannii Infections Disclosed a Faster Increase in OECD Countries. Emerg Microbes Infect 7 (1), 31 Wang, S., Li, Z., Liu, Y., Feng, G., Zheng, J., Yuan, Z., and Zhang, X. (2018) Activatable Photoacoustic and Fluorescent Probe of Nitric Oxide for Cellular and in Vivo Imaging. Sensors and Actuators B: Chemical 267, 403-411 Gao, D. Y., Ji, X., Wang, J. L., Wang, Y. T., Li, D. L., Liu, Y. B., Chang, K. W., Qu, J. L., Zheng, J., and Yuan, Z. (2018) Engineering a Protein-Based Nanoplatform as an Antibacterial Agent for Light Activated Dual-Modal Photothermal and Photodynamic Therapy of Infection in Both the NIR I and II Windows. J Mater Chem B 6 (5), 732-739 Li, S. K., Jiang, N., Zhao, W. X., Ding, Y. F., Zheng, Y., Wang, L. H., Zheng, J., and Wang, R. B. (2017) An Eco-Friendly in Situ Activatable Antibiotic Via Cucurbit[8]Uril-Mediated Supramolecular Crosslinking of Branched Polyethylenimine. Chem Commun 53 (43), 5870-5873 Kuok, K. I., In Ng, P. C., Ji, X., Wang, C. M., Yew, W. W., Chan, D., Zheng, J., Lee, S., and Wang, R. B. (2017) Supramolecular Strategy for Reducing the Cardiotoxicity of Bedaquiline without Compromising Its Antimycobacterial Efficacy. Food Chem Toxicol 119, 425-429 Zhang, M., Kong, X., Zheng, J., Wan, J. B., Wang, Y., Hu, Y., and Shao, R. (2016) Research and Development of Antibiotics: Insights from Patents and Citation Network. Expert Opin Ther Pat 26 (5), 617-627 Moradigaravand, D., Grandjean, L., Martinez, E., Li, H., Zheng, J., Coronel, J., Moore, D., Torok, M. E., Sintchenko, V., Huang, H., Javid, B., Parkhill, J., Peacock, S. J., and Koser, C. U. (2016) dfrA thyA Double Deletion in para-Aminosalicylic Acid-Resistant Mycobacterium Tuberculosis Beijing Strains. Antimicrob Agents Chemother 60 (6), 3864-3867 Li, S., Chan, J. Y., Li, Y., Bardelang, D., Zheng, J., Yew, W. W., Chan, D. P., Lee, S. M., and Wang, R. (2016) Complexation of Clofazimine by Macrocyclic Cucurbit[7]Uril Reduced Its Cardiotoxicity without Affecting the Antimycobacterial Efficacy. Org Biomol Chem 14 (31), 7563-7569 Zheng, J., Rubin, E. J., Bifani, P., Mathys, V., Lim, V., Au, M., Jang, J., Nam, J., Dick, T., Walker, J. R., Pethe, K., and Camacho, L. R. (2013) para-Aminosalicylic Acid Is a Prodrug Targeting Dihydrofolate Reductase in Mycobacterium Tuberculosis. J Biol Chem 288 (32), 23447-23456 Mak, P. A., Rao, S. P., Ping Tan, M., Lin, X., Chyba, J., Tay, J., Ng, S. H., Tan, B. H., Cherian, J., Duraiswamy, J., Bifani, P., Lim, V., Lee, B. H., Ling Ma, N., Beer, D., Thayalan, P., Kuhen, K., Chatterjee, A., Supek, F., Glynne, R., Zheng, J., Boshoff, H. I., Barry, C. E., 3rd, Dick, T., Pethe, K., and Camacho, L. R. (2012) A High-Throughput Screen to Identify Inhibitors of ATP Homeostasis in Non-Replicating Mycobacterium Tuberculosis. Acs Chem Biol 7 (7), 1190-1197 Zheng, J., Ho, B., and Mekalanos, J. J. (2011) Genetic Analysis of Anti-Amoebae and Anti-Bacterial Activities of the Type VI Secretion System in Vibrio Cholerae. PLoS One 6 (8), e23876 Schmitt, E. K., Riwanto, M., Sambandamurthy, V., Roggo, S., Miault, C., Zwingelstein, C., Krastel, P., Noble, C., Beer, D., Rao, S. P., Au, M., Niyomrattanakit, P., Lim, V., Zheng, J., Jeffery, D., Pethe, K., and Camacho, L. R. (2011) The Natural Product Cyclomarin Kills Mycobacterium Tuberculosis by Targeting the ClpC1 Subunit of the Caseinolytic Protease. Angew Chem Int Ed Engl 50 (26), 5889-5891 Zheng, J., Shin, O. S., Cameron, D. E., and Mekalanos, J. J. (2010) Quorum Sensing and a Global Regulator TsrA Control Expression of Type VI Secretion and Virulence in Vibrio Cholerae. Proc Natl Acad Sci U S A 107 (49), 21128-21133 Xie, H. X., Yu, H. B., Zheng, J., Nie, P., Foster, L. J., Mok, Y. K., Finlay, B. B., and Leung, K. Y. (2010) EseG, an Effector of the Type III Secretion System of Edwardsiella Tarda, Triggers Microtubule Destabilization. Infect Immun 78 (12), 5011-5021 Jobichen, C., Chakraborty, S., Li, M., Zheng, J., Joseph, L., Mok, Y. K., Leung, K. Y., and Sivaraman, J. (2010) Structural Basis for the Secretion of EvpC: A Key Type VI Secretion System Protein from Edwardsiella Tarda. PLoS One 5 (9), e12910 Zheng, J., Li, N., Tan, Y. P., Sivaraman, J., Mok, Y. K., Mo, Z. L., and Leung, K. Y. (2007) EscC Is a Chaperone for the Edwardsiella Tarda Type III Secretion System Putative Translocon Components EseB and EseD. Microbiology 153 (Pt 6), 1953-1962 Zheng, J., and Leung, K. Y. (2007) Dissection of a Type VI Secretion System in Edwardsiella Tarda. Mol Microbiol 66 (5), 1192-1206 Zheng, J., Tung, S. L., and Leung, K. Y. (2005) Regulation of a Type III and a Putative Secretion System in Edwardsiella Tarda by EsrC Is under the Control of a Two-Component System, EsrA-EsrB. Infect Immun 73 (7), 4127-4137 Tan, Y. P., Zheng, J., Tung, S. L., Rosenshine, I., and Leung, K. Y. (2005) Role of Type III Secretion in Edwardsiella Tarda Virulence. Microbiology 151 (Pt 7), 2301-2313