何玉财：男，博士，教授、硕导，主要从事生物质能源、生物基产品与生物催化合成研究。

GROUPMEMBERS（课题组成员）： 2012届硕士研究生：周琼（联合培养）；

2013届硕士研究生：夏东琴（硕士研究生国奖获得者）（联合培养）；

2014届硕士研究生：杨振兴，龚磊，潘雪鹤（联合培养）；

2013级硕士研究生：陶志成（硕士研究生国奖获得者），张丹平（扬巴奖学金获得者）；

2014级硕士研究生：刘峰（硕士研究生国奖获得者，两次），丁云；

2015级硕士研究生：种刚刚；

2016级硕士研究生：姜春霞（硕士研究生国奖获得者，两次），薛新霞（硕士研究生国奖获得者），王冰倩，顾婷

2017级硕士研究生：黄晓君，张瑞钦；

2018级硕士研究生：李园园，朱旋，阳东

RESEARCHEXPERIENCE（研究兴趣）：

1）Bioconversionofcellulosicmaterialstofuelethanolandothervalue-addedproductsandchemicals

SpecificResearchEmphasis:a).Pretreatmentofcellulosicmaterialsandrelatedreactionsystemdesignandkineticsstudy;b).Investigationofthesynergeticeffectsbetweencellulaseandhemicellulaseduringenzymatichydrolysisanditsmechanics;c).Fermentationofsugarsforproductionofbioethanolandotherfuels;d).Investigationoftherelationshipbetweenpretreatmentandenzymatichydrolysisandmethodstoimprovetheoverallcelluloseandhemicellulosesconversion;e).Screeningofcellulase-producingstrainanditsfermentationoptimization.

Biocatalysis

2）Biosynthesisofopticallypurechiralcompoundsorotherimportantintermediateswithextremelyhighvaluesforpharmaceuticalsandagrochemicals

SpecificResearchEmphasis:a).Screeninganddevelopmentofalotofhighlyenantioselelctivebiocatalystsbysubstrate-orientedscreening,suchasnitrilases,esterasesandoxidoreductases;b).Forproductionofhighlyvalue-addedchiralsynthonsorotherintermediates,manyefficientbiotransformationprocesses,eitherbymanipulatingthemediumcomposition(e.g.,additionoforganiccosolvents,metalions,inducers,orsurfactantslikeTween-80)andthereactionconditions,orbyoptimizingthebioreactorconfigurationoritsoperationmode,resultinginsignificantenhancementofselectivityandproductivity;c).Immobilizationtechnologyisusedforenhancingoperationstability;d).Cloneandoverexpressionofnitrilaseandreductase.

ACHIEVEMENTS(成果)：

已发表SCI文章70余篇（其中在GreenChemistry、BioresourceTechnology和IndustrialCropsandProducts等杂志发表JCR一区26篇，在ACSSustainableChemistry&Engineering、AppliedMicrobiologyandBiotechnology、CarbohydratePolymers、JournalofBiotechnology和JournalofIndustrialMicrobiologyandBiotechnology等杂志发表JCR二区12篇，在AppliedBiochemistryandBiotechnology、BioprocessandBiosystemsEngineering、BiotechnologyandBioprocessEngineering、Bioengineered、BiotechnologyLetters、Biology&Environment:ProceedingsoftheRoyalIrishAcademy、ChemicalPapers、CatalysisCommunications、ChineseChemicalLetters、DesalinationandWaterTreatment、JournaloftheAmericanOilChemists、JournalofMolecularCatalysisB:Enzymatic、ProcessBiochemistry等杂志发表30余篇），发表EI收录的文章11篇，申请中国专利近25项；引用次数1600次以上。

JCR一区：（26篇）SCI

1.One-potchemo-enzymaticconversionofD-xylosetofurfuralcoholbysequentialdehydrationwithoxalicacidplustin-basedsolidacidandbioreductionwithwhole-cells.BioresourceTechnology,2018,268:292-299.

2.One-potco-catalysisofcorncobwithdilutehydrochloricacidandtin-basedsolidacidfortheenhancementoffurfuralproduction.BioresourceTechnology,2018,268:315-322.

3.Chemo-enzymaticsynthesisoffurfuralcoholfromchestnutshellhydrolysatebyasequentialacid-catalyzeddehydrationundermicrowaveandEscherichiacoliCCZU-Y10whole-cellsconversion.BioresourceTechnology,2018,262:52-58.

4.Microbiallipidproductionfromenzymatichydrolysateofcornstoverpretreatedbycombiningwithbiologicalpretreatmentandalkalicsaltsoaking.IndustrialCropsandProducts,2018,124:487-494.

5.Enhancedbioreductionsynthesisofethyl(R)-4-chloro-3-hydroybutanoatebyalkalicsaltpretreatment.BioresourceTechnology,2018,261:196-205.

6.Biologicalsynthesisof2,5-bis(hydroxymethyl)furanfrombiomass-derived5-hydroxymethylfurfuralbyE.coliCCZU-K14wholecells.BioresourceTechnology,2018,247:1215-1220

7.One-potconversionofbiomass-derivedxylosetofurfuralcoholbyachemo-enzymaticsequentialacid-catalyzeddehydrationandbioreduction.GreenChemistry.2017,19:3844–3850.

8.One-potchemo-enzymaticsynthesisoffurfuralcoholfromxylose.BioresourceTechnology2017,238:698-705.

9.Effectiveenzymaticinsitusaccharificationofbambooshootshellpretreatedbydilutealkalicsaltssodiumhypochlorite/sodiumsulfidepretreatmentundertheautoclavesystem.BioresourceTechnology2017,241:726-734.

10.Chemical-enzymaticconversionofcorncob-derivedxylosetofurfuralcoholbythetandemcatalysiswithSO42-/SnO2-KaolineandE.coliCCZU-T15cellsintoluene–watermedia.BioresourceTechnology2017,245:841-849.

11.BiologicalconversionoftheaqueouswastesfromhydrothermalliquefactionofalgaeandpinewoodbyRhodococci.BioresourceTechnology2017,224:457-464.

12.ImprovingenzymaticsaccharificationofbambooshootshellbyalkalisaltpretreatmentwithH2O2.BioresourceTechnology2016,201:230-236.

13.Acombinedsodiumphosphateandsodiumsulfidepretreatmentforenhancedenzymaticdigestibilityanddelignificationofcornstover.BioresourceTechnology2016,218:209-216.

14.Effectiveasymmetricbioreductionofethyl4-chloro-3-oxobutanoatetoethyl(R)-4-chloro-3-hydroxybutanoatebyrecombinantE.coliCCZU-A13in[Bmim]PF6–hydrolyzatemedia.BioresourceTechnology2016,214:414-418.

15.Effectivepretreatmentofsugarcanebagassewithcombinationpretreatmentanditshydrolyzatesasreactionmediaforthebiosynthesisofethyl(S)-4-chloro-3-hydroxybutanoatebywholecellsofE.coliCCZU-K14.BioresourceTechnology2016,211:720-726.

16.Enzymaticinsitusaccharificationofchestnutshellwithhighionicliquid-tolerantcellulasesfromGalactomycessp.CCZU11-1inabiocompatibleionicliquid-cellulasemedia.BioresourceTechnology2016,201:133-139.

17.EffectiveenzymaticsaccharificationofdiluteNaOHextractionofchestnutshellpretreatedbyacidifiedaqueousethyleneglycolmedia.IndustrialCropsandProducts2016,81:129-138

18.Significantlyimprovingenzymaticsaccharificationofhighcrystallinityindex’scornstoverbycombiningionicliquid[Bmim]Cl–HCl–watermediawithdiluteNaOHpretreatment.BioresourceTechnology2015,189:421-425.

19.Improvedbiosynthesisofethyl(S)-4-chloro-3-hydroxybutanoatebyaddingL-glutamineplusglycineinsteadofNAD+inβ-cyclodextrin−watersystem.BioresourceTechnology2015,182:98-102.

20.EnhancementofenzymaticsaccharificationofcornstoverwithsequentialFentonpretreatmentanddiluteNaOHextraction.BioresourceTechnology2015,193:324-330.

21.Discoveryofareductase-producingstrainrecombinantE.coliCCZU-A13usingcolorimetricscreeninganditswholecell-catalyzedbiosynthesisofethyl(R)-4-chloro-3-hydroxybutanoate.BioresourceTechnology,2014,172:342-348.

22.Highlyefficientsynthesisofethyl(S)-4-chloro-3-hydroxybutanoateanditsderivativesbyarobustNADH-dependentreductasefromE.coliCCZU-K14.BioresourceTechnology,2014,161:461-464.

23.EnzymaticsaccharificationofsugarcanebaggagebyN-methylmorpholine-N-oxide-tolerantcellulasefromanewlyisolatedGalactomycessp.CCZU11-1.BioresourceTechnology,2013,135:18-22.

24.BiosynthesisofbenzoylformicacidfrombenzoylcyanidebyanewlyisolatedRhodococcussp.CCZU10-1intoluene-waterbiphasicsystem.BioresourceTechnology,2012,115:88-95.

25.Improvingenzymatichydrolysisofwheatstrawusingionicliquid1-ethyl-3-methylimidazoliumdiethylphosphatepretreatment.BioresourceTechnology,2010,100(14):3570-3575.

26.ABronstedacidicionicliquidasanefficientandenvironmentalbenigncatalystforbiodieselsynthesisfromfreefattyacidsandachohols.BioresourceTechnology,2009,100(19):4368-4373.

JCR二区:（12篇）SCI

27.LipidproductionfromdilutealkalicornstoverligninbyRhodococcusstrains.ACSSustainableChem.Eng.,2017,5:2302-2311.

28.EnzymaticinsitusaccharificationofsugarcanebagassepretreatedwithlowloadingofalkalicsaltsNa2SO3/Na3PO4byautoclaving.JournalofBiotechnology2017,259:73-82.

29.EfficientbiosynthesisofrarenaturalproductscopolamineusingE.colicellsexpressingaS14P/K97Amutantofhyoscyamine6β-hydroxylaseAaH6H.JournalofBiotechnology2015,211,123-129.

30.Effectivebiosynthesisofethyl(R)-4-chloro-3-hydroxybutanoatebysupplementationofL-glutamine,D-xyloseandβ-cyclodextrininn-butylacetate–watermedia.JournalofBiotechnology2015,203:62-67.

31.RemovalofCu2+fromaqueoussolutionsbythenovelmodifiedbagassepulpcellulose:Kinetics,isothermandmechanism.CarbohydratePolymers2015,129:115-126.

32.Investigationofanovelacidcatalyzedionicliquidpretreatmentmethodtoimprovebiomassenzymatichydrolysisconversion.AppliedMicrobiologyandBiotechnology,2014,98:5275-5286.

33.Highlyenantioselectiveoxidationofphenylmethylsulfideanditsderivativesintoopticallypure(S)-sulfoxideswithRhodococcussp.CCZU10-1inann-octane-waterbiphasicsystem.AppliedMicrobiologyandBiotechnology,2013,97:10329-10337.

34.Highlyenantioselectiveoxidationofracemicphenyl-1,2-ethanedioltoopticallypure(R)-(-)-mandelicacidbyanewlyisolatedBrevibacteriumlutescensCCZU12-1.AppliedMicrobiologyandBiotechnology,2013,97:7185-7194.

35.DegradationofthenovelherbicideZJ0273byAmycolatopsissp.M3-1isolatedfromsoil.AppliedMicrobiologyandBiotechnology,2012,96(5),1371-1379.

36.Ahigh-throughputscreeningstrategyfornitrile-hydrolyzingenzymesbasedonferrichydroxamatespectrophotometry.AppliedMicrobiologyandBiotechnology,2011,89:817-823.

37.Significantenhancementof(R)-mandelicacidproductionbyrelievingsubstrateinhibitionofrecombinantnitrilaseintoluene-waterbiphasicsystem.JournalofBiotechnology,2011,152:24-29.

38.Biocatalyticsynthesisof(R)-(-)-mandelicacidfromracemicmandelonitrilebycetyl-trimethylammoniumbromide-permeabilizedcellsofAlcaligenesfaecalisECU0401.JournalofIndustrialMicrobiologyandBiotechnology,2010,377:741-750.