Ph.D., The George Washington University, 2001

Physical Chemistry Mass spectrometry Lipidomics Chemical Biology Biophysical Chemistry Bioanalytical Chemistry Analytical Chemistry

Bioanalytical and Biophysical Chemistry Our research focuses on the design, construction, and application of advanced technologies for structural mass spectrometry, in particular, for studies in structural proteomics, systems biology, and biophysics. To identify and structurally characterize biomolecules from complex samples, we perform rapid (µs-ms) two-dimensional gas-phase separations using ion mobility-mass spectrometry (IM-MS) techniques. IM-MS provides separations on the basis of apparent surface area (ion-neutral collision cross section) and mass-to-charge (m/z), respectively. Biomolecular structural information is interpreted by comparing experimentally obtained collision cross-sections in the context of those obtained via molecular dynamics simulations.

Lamberson, C. R., Xu, L., Muchalski, H., Montenegro-Burke, J. R., Shmanai, V. V., Bekish, A. V., McLean, J. A., Clarke, C. F., Shchepinov, M. S., Porter, N. A. Unusual kinetic isotope effects of deuterium reinforced polyunsaturated fatty acids in tocopherol-mediated free radical chain oxidations. Journal of the American Chemical Society. 2014, 136 (3): 838-41. May, J. C., Goodwin, C. R., Lareau, N. M., Leaptrot, K. L., Morris, C. B., Kurulugama, R. T., Mordehai, A., Klein, C., Barry, W., Darland, E., Overney, G., Imatani, K., Stafford, G. C., Fjeldsted, J. C., McLean, J. A. Conformational ordering of biomolecules in the gas phase: nitrogen collision cross sections measured on a prototype high resolution drift tube ion mobility-mass spectrometer. Analytical Chemistry. 2014, 86 (4): 2107-16. Hines, K. M., Ashfaq, S., Davidson, J. M., Opalenik, S. R., Wikswo, J. P., McLean, J. A. Biomolecular Signatures of Diabetic Wound Healing by Structural Mass Spectrometry. Analytical Chemistry. 2013, 85 (7): 3651-3659. Derewacz, D. K., Goodwin, C. R., McNees, C. R., McLean, J. A., Bachmann, B. O. Antimicrobial drug resistance affects broad changes in metabolomic phenotype in addition to secondary metabolism. Proceedings of The National Academy of Sciences of The United States of America. 2013, 110 (6): 2336-2341. Harkness, K. M., Tang, Y., Dass, A., Pan, J., Kothalawala, N., Reddy, V. J., Cliffel, D. E., Demeler, B., Stellacci, F., Bakr, O. M., McLean, J. A. Ag-44(SR)(30)(4-): a silver-thiolate superatom complex. Nanoscale. 2012, 4 (14): 4269-4274. Matusch, A., Fenn, L. S., Depboylu, C., Klietz, M., Strohmer, S., McLean, J. A., Becker, J. S. Combined Elemental and Biomolecular Mass Spectrometry Imaging for Probing the Inventory of Tissue at a Micrometer Scale. Analytical Chemistry. 2012, 84 (7): 3170-3178. Harkness, K. M., Turner, B. N., Agrawal, A. C., Zhang, Y. B., McLean, J. A., Cliffel, D. E. Biomimetic monolayer-protected gold nanoparticles for immunorecognition. Nanoscale. 2012, 4 (13): 3843-3851. Goodwin, C. R., Fenn, L. S., Derewacz, D. K., Bachmann, B. O., McLean, J. A. Structural Mass Spectrometry: Rapid Methods for Separation and Analysis of Peptide Natural Products. Journal of Natural Products. 2012, 75 (1): 48-53. Harkness, K. M., Balinski, B., Mclean, J. A., Cliffel, D. E. Nanoscale Phase Segregation of Mixed Thiolates on Gold Nanoparticles. Angewandte Chemie-International Edition. 2011, 50 (45): 10554-10559. Kerr, T. J., Gant-Branum, R. L., Mclean, J. A. Multiplexed analysis of peptide functionality using lanthanide-based structural shift reagents. International Journal of Mass Spectrometry. 2011, 307 (1-3): 28-32. Kliman, M., May, J. C., Mclean, J. A. Lipid analysis and lipidomics by structurally selective ion mobility-mass spectrometry. Biochimica ET Biophysica ACTA-Molecular And Cell Biology Of Lipids. 2011, 1811 (11): 935-945. Lareau, N. M., Fenn, L. S.,Goodwin, C. R., Bachmann, B. O., Mclean, J. A. Exploring conformation space for natural product discovery. Abstracts of Papers of The American Chemical Society. 2011, 241 (0): 165-CHED. Fenn, L. S., Mclean, J. A. Structural resolution of carbohydrate positional and structural isomers based on gas-phase ion mobility-mass spectrometry. Physical Chemistry Chemical Physics. 2011, 13 (6): 2196-205. Fenn, L. S., Mclean, J. A. Structural Characterization of Carbohydrates and Glycoproteins using Ion Mobility-Mass Spectrometry. Glycobiology. 2010, 20 (11): 1474-1474. Kerr, T. J., McLean, J. A. Peptide quantitation using primary amine selective metal chelation labels for mass spectrometry. Chemical Communications. 2010, 46 (30): 5479-5481. Ridenour, W.B., Kliman, M., McLean, J.A., Caprioli, R.M. Structural Characterization of Phospholipids and Peptides Directly from Tissue Sections by MALDI Traveling-Wave Ion Mobility-Mass Spectrometry. Analytical Chemistry. 2010, 82 (5): 1881-1889. Gant-Branum, R.L., Broussard, J.A., Mahsut, A., Webb, D.J., McLean, J.A. Identification of Phosphorylation Sites within the Signaling Adaptor APPL1 by Mass Spectrometry. Journal of Proteome Research. 2010, 9 (3): 1541-1548. Harkness, K.M., Fenn, L.S., Cliffel, D.E., McLean, J.A. Surface Fragmentation of Complexes from Thiolate Protected Gold Nanoparticles by Ion Mobility-Mass Spectrometry. Analytical Chemistry. 2010, 82 (7): 3061-3066.

Sundarapandian, S., May, J.C., McLean, J.A. Dual Source Ion Mobility-Mass Spectrometer for Direct Comparison of Electrospray Ionization and MALDI Collision Cross Section Measurements. Analytical Chemistry. 2010, 82 (8): 3247-3254.