Biography Download Curriculum Vitae Dr. Rosen is the Chair of the Department of Biophysics at UT Southwestern and an Investigator of the Howard Hughes Medical Institute. Prior to moving to UT Southwestern in 2001 he was a member of the Cellular Biochemistry and Biophysics Program at the Memorial Sloan-Kettering Cancer Center in New York City. He received undergraduate degrees in chemistry and in chemical engineering from the University of Michigan in 1987, and then spent a year in Alan Battersby’s lab in the Department of Chemistry at the University of Cambridge as a Winston Churchill Foundation Scholar. He received his Ph.D. in chemistry from Harvard University in 1993 under the direction of Stuart Schreiber, where he studied the structure and function of the FK506 binding protein, FKBP12. He was a Damon Runyon-Walter Winchell post-doctoral fellow in the laboratories of Tony Pawson and Lewis Kay at the University of Toronto, where he studied regulation of the signaling adaptor protein, Crk, and developed methods of selective methyl group labeling of proteins for NMR spectroscopy. The Rosen lab website can be found at: www.utsouthwestern.edu/labs/rosen/ Education Undergraduate University of Michigan-Ann Arbor (1987), Chemistry Undergraduate University of Michigan-Ann Arbor (1987), Chemical Engineering Graduate School Harvard University (1993), Organic Chemistry Honors & Awards Mar Nell and F. Andrew Bell Distinguished Chair in Biochemistry (2009) Carolyn R. Bacon Professorship in Medical Science and Education (2006) Edith & Peter O’Donnell Award from the Texas Academy of Medicine, Engineering and Science (2006) Howard Hughes Medical Institute (2005) Sidney Kimmel Scholar Award (1998) Beckman Young Investigator (1997) Presidential Early Career Award for Scientists and Engineers (PECASE) (1997) Winston Churchill Scholar (1987)

Research Interest biochemistry cell biology of the actin cytoskeleton polymer physics/chemistry structural biology

Chia PH, Chen B, Li P, Rosen MK, Shen K. Local F-actin Network Links Synapse Formation and Axon Branching. Cell. 2014 Jan 16;156(1-2):208-20. PMID: 24439377 Chen B, Brinkmann K, Chen Z, Pak CW, Liao Y, Shi S, Henry L, Grishin NV, Bogdan S, Rosen MK. The WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton. Cell. 2014 Jan 16;156(1-2):195-207. PMID: 24439376 Zahm JA, Padrick SB, Chen Z, Pak CW, Yunus AA, Henry L, Tomchick DR, Chen Z, Rosen MK. The bacterial effector VopL organizes actin into filament-like structures. Cell. 2013 Oct 10;155(2):423-34. PMID: 24120140 Smith BA, Padrick SB, Doolittle LK, Daugherty-Clarke K, Corrêa IR Jr, Xu MQ, Goode BL, Rosen MK, Gelles J. Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation. Elife. 2013 Sep 3;2:e01008. PMID: 24015360 Hao YH, Doyle JM, Ramanathan S, Gomez TS, Jia D, Xu M, Chen ZJ, Billadeau DD, Rosen MK, Potts PR., Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitination, Cell, 2013, 152(5):1051-64. PMCID: PMC3640276 Doolittle LK, Rosen MK, Padrick SB., Measurement and analysis of in vitro actin polymerization, Methods Mol Biol, 2013, 1046:273-93. PMID: 23868594. Doolittle LK, Rosen MK, Padrick SB., Purification of Arp2/3 Complex from Saccharomyces cerevisiae, Methods Mol Biol, 2013, 1046:251-71. PMID: 23868593. Doolittle LK, Rosen MK, Padrick SB., Purification of native arp2/3 complex from bovine thymus, Methods Mol Biol, 2013, 1046:231-50. PMID: 23868592. Li, P., Banjade, S., Cheng, H.- C., Kim, S., Chen, B., Guo, L., Llaguno, M., Hollingsworth, J. V., King, D. S., Banani, S. F., Russo, P. S., Jiang, Q-. X., Nixon, B. T., Rosen, M.K., Phase Transitions in the Assembly of Multi-Valent Signaling Proteins, Nature, 2012, 483, 336-40. PMCID-PMC3343696. Jia, D., Gomez, T. S., Billadeau, D. D., Rosen, M.K., Multiple Repeat Elements Within the FAM21 Tail Link the WASH Actin Regulatory Complex to the Retromer, Mol. Biol. Cell, 2012, 23(12), 2352-61. PMCID-PMC3374753. Rizo, J., Rosen, M.K., Gardner, K. H., Enlightening Molecular Mechanisms through Study of Protein Interactions, J. Molec. Cell Biol., 2012, doi, "10.1093/jmcb/mjs036. Padrick, S. B., Doolittle, L. K., Brautigam, C. A., King, D. S., Rosen, M.K., Arp2/3 Complex is Bound and Activated by Two VCA Peptides, Proc. Natl. Acad. Sci. USA,2011, 108(33), E472-479. PMCID-PMC3158169. Yu, B., Cheng, H.- C., Brautigam, C. A., Tomchick, D. R., Rosen, M.K., Mechanism of Actin Filament Nucleation by the Bacterial Effector VopL, Nat. Struct. Mol. Biol., 2011, 18(9), 1068-1074. PMCID-PMC3168117. Li, P., Martins, I. R. S., Rosen, M.K., The Feasibility of Parameterizing Four-State Equilibria Using Relaxation Dispersion Measurements, J. Biomol. NMR, 2011, 51, 57-70. PMCID-PMC3229927. Yu, B., Martins, I. R. S., Li, P., Amarasinghe, G. K., Umetani, J., Fernandez-Zapico, M., Billadeau, D. D., Machius, M., Tomchick, D. R., Rosen, M.K., Structural and Energetic Mechanisms of Cooperative Autoinhibition and Activation of Vav1, Cell, 2010, 140, 246-256. PMCID-PMC2825156. Padrick, S. B., Rosen, M.K., Physical Mechanisms of Signal Integration by WASP Family Proteins, Ann. Rev. Biochemistry, 2010, 79, 707-735. PMCID-PMC3017724. Westerberg, L. S., Meelu, P., Baptista, M., Adamovich, D. A., Cotta-de-Almeida, V., Seed, B., Rosen, M.K., Vandenberghe, P., Thrasher, A. J., Klein, C., Alt, F. W., Snapper, S. B., Activating WASP Mutations Associated With X-Linked Neutropenia Result in Enhanced Actin Polymerization, Altered Cytoskeletal Responses and Genomic Instability in Lymphocytes, J. Exp. Med., 2010, 207(6), 1145-1152. PMCID-PMC2882832. Jia, D., Gomez, T. S., Metlagel, Z., Umetani, J., Otwinowski, Z., Rosen, M.K., Billadeau, D. D., WASH and WAVE Actin Regulators are Controlled by Analogous Structurally Related Complexes, Proc. Natl. Acad. Sci. USA, 2010, 107(23), 10442-10447. PMCID-PMC2890800. Strickland, D., Yao, X., Gawlak, G., Rosen, M.K.*, Gardner, K. H.*, Sosnick, T. R,.* Rationally increasing the dynamic range of genetically-encoded, optically-controlled proteins, Nat. Methods., 2010, 7(8), 623-626. PMCID-PMC2914111.

Padrick, S. B., Deka, R. K., Chuang, J. L., Wynn, R. M., Chuang, D. T., Norgard, M. V., Rosen, M.K., Brautigam, C. A., Determination of Protein Complex Stoichiometry Through Multisignal Sedimentation Velocity Experiments, Anal. Biochem., 2010, 407(1), 89-103. PMCID-PMC3089910.