B.S., Chemistry, 1985, University of Wisconsin at Eau Claire Ph.D., Analytical Chemistry, 1992, Purdue University Postdoctoral Fellow, Colorado School of Mines

Analytical Chemistry

The area of research of the Basile Group at the University of Wyoming is Analytical Chemistry with a focus on the application of Mass Spectrometry to analyze a variety of biological and environmental samples. Specifically, our research is aimed at developing rapid biological sample preparation techniques suitable for proteomic measurements that can be implemented in field-portable instrumentation. These techniques are based on the use of non-enzymatic steps to lyse/digest biological samples to a stage that is suitable for MS analysis. Our laboratory pioneered the use of microwave heating for non-enzymatic digestion of proteins at aspartic acid and disulfide bonds and Thermal Degradation/Digestion that reduce digestion times from hours to seconds.

Research conducted by the Basile Group also focuses on the development of broad-spectrum techniques, both methodology and instrumentation, for the analysis of complex mixtures in biological (metabolomics) and environmental (coal) samples. More information about our current research can be found in our group website.

Rajendra Mahat, Wesley Rodgers, Franco Basile, Microwave Radiation Heating in Pressurized Vessels for the Rapid Extraction of Coal Samples for Broad Spectrum GC–MS Analysis, Energy & Fuels, 2014, 28, 6326-6335 journal link

Lu, L. and F. Basile, Thermal decomposition of phosphorylated peptides in the condensed phase as studied by mass spectrometry. Journal of Analytical and Applied Pyrolysis, 2013, 104, 412-417 journal link

Gwendoline M. Toh-Boyo, Shaun S. Wulff, Franco Basile, Comparison of Sample Preparation Methods and Evaluation of Intra and Intersample Reproducibility in Bacteria MALDI-MS Profiling, Anal. Chem. 2012, 84, 9971-9980 journal link

Basile, F., Zhang, S., Kandar, S., Lu, L., Mass Spectrometry Characterization of the Thermal Decomposition/Digestion (TDD) at Cysteine in Peptides and Proteins in the Condensed Phase, J. Am. Soc. Mass Spectrom., 22 (2011) 1926-1940

Chenglin Liu, Franco Basile, Intermolecular condensation products formed during the pyrolysis of peptides, J. Analytical and Applied Pyrolysis, 92 (2011) 217-223

Basile, F., Hauser, N. J., Rapid Online Non-Enzymatic Protein Digestion Combining Microwave Heating Acid Hydrolysis and Electrochemical Oxidation, Anal. Chem., 2011, 83, 359-367. Journal link

Basile, F.; Sibray, T.; Belisle, J.; Bowen, R., Analysis of Lipids from Crude Lung Tissue Extracts by Desorption Electrospray Ionization-MS and Pattern Recognition. Anal. Biochem. 2011, 408, 289-296

Basile, F.; Zhang, S.; Shin, Y.-S.; Drolet, B., Atmospheric pressure-thermal desorption (AP-TD)/electrospray ionization-mass spectrometry for the rapid analysis of Bacillus spores. Analyst 2010, 135, (4), 797-803.

Hauser, N.J. and F. Basile, On-line Microwave D-Cleavage LC-ESI-MS/MS of Intact Proteins: Site-Specific Cleavages at Aspartic Acid Residues and Disulfide Bonds. Journal of Proteome Research, 2008. 7: p. 1012-1026. PubMed abstract, Journal link

Hauser, N., H. Han, S.A. McLuckey, and F. Basile, Electron Transfer Dissociation of Peptides Generated by Microwave D-cleavage Digestion of Proteins. J. Proteome Res., 2008. 7: p. 1867-1872. PubMed abstract

Zhang, S., Y.-S. Shin, R. Mayer, and F. Basile, On-probe pyrolysis desorption electrospray ionization (DESI) mass spectrometry for the analysis of non-volatile pyrolysis products. Journal of Analytical and Applied Pyrolysis, 2007. 80(2): p. 353-359. Journal link

Zhang, S. and F. Basile, Site-Specific Pyrolysis-Induced Cleavage at Aspartic Acid Residue in Peptides and Proteins. J. Proteome Res., 2007. 6(5): p. 1700-1704. PubMed abstract

Shin, Y.-S., B. Drolet, R. Mayer, K. Dolence, and F. Basile, Desorption Electrospray Ionization-Mass Spectrometry of Proteins. Analytical Chemistry, 2007. 79(9): p. 3514-3518. PubMed abstract, Journal link

Meetani, M.A., Y.-S. Shin, S. Zhang, R. Mayer, and F. Basile, Desorption electrospray ionization mass spectrometry of intact bacteria. Journal of Mass Spectrometry, 2007. 42(9): p. 1186-1193. PubMed abstract, Journal link

Basile, F., G.E. Kassalainen, and S.K.R. Williams, Interface for Direct and Continuous Sample-Matrix Deposition onto a MALDI Probe for Polymer Analysis by Thermal Field Flow Fractionation and Off-Line MALDI-MS. Analytical Chemistry, 2005. 77(9): p. 3008-3012. PubMed abstract, Journal link

Havey, C.D., F. Basile, C. Mowry, and K.J. Voorhees, Evaluation of a micro-fabricated pyrolyzer for the detection of Bacillus anthracis spores. Journal of Analytical and Applied Pyrolysis, 2004. 72(1): p. 55-61. Journal link

Fung, K.Y.C., S. Askovic, F. Basile, and M.W. Duncan, A simple and inexpensive approach to interfacing high-performance liquid chromatography and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Proteomics, 2004. 4(10): p. 3121-3127. PubMed abstract

Basile, F., I. Ferrer, E.T. Furlong, and K.J. Voorhees, Simultaneous multiple substrate tag detection with ESI-ion trap MS for in vivo bacterial enzyme activity profiling. Analytical Chemistry, 2002. 74(16): p. 4290-4293. PubMed abstract

Madonna, A.J., F. Basile, E. Furlong, and K.J. Voorhees, Detection of bacteria from biological mixtures using immunomagnetic separation combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry, 2001. 15(13): p. 1068-1074.

Madonna, A.J., F. Basile, I. Ferrer, M.A. Meetani, J.C. Rees, and K.J. Voorhees, On-probe sample pretreatment for the detection of proteins above 15 KDa from whole cell bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry, 2000. 14(23): p. 2220-2229. Journal link