Senior Lecturer in Virology (2016) University of Surrey, Guildford, UK Viral RNA, Translation, Virus-host interactions Lecturer in Virology (2009) University of Surrey, Guildford, UK Post Doctoral Fellow (2007-2009) Université Paris Descartes, Paris, Fr Dr Sargueil group: Viral RNA, HIV, translation Post Doctoral Fellow (2004-2007) Laboratory of Molecular Biology, MRC Cambridge, UK Dr Lukavsky group: Viral RNA, HCV, translation Ph.D in Protein Engineering (2003) Université Paris XI, Institut de Chimie des Substances Naturelles, Gif, Fr Dr Guittet group: RNA, NMR structure and function, Master in Molecular Biophysic (2000) Université Paris VI, Fr

Our main research interests are the regulation of gene expression and translational control during viral infection. We are trying to address fundamental questions at the interface between microbiology and RNA biology by asking how microbial genomes take control of the infected host protein synthesis machinery and how this impacts on the host translational landscape at the molecular and cellular level. My previous research has focused on the molecular mechanisms by which viral RNAs elements regulate protein synthesis, focusing on Hepatitis C and Human Immunodeficiency Viruses. To decipher the mechanism by which internal ribosome entry site (IRES) interact with the translation machinery, I set up new biochemical tools to study translation in vitro and unravelled the pathway of 80S ribosome formation mediated by the HCV IRES and shed light on a new, original, translation initiation mechanism used by lentiviruses to produce several isoforms of the Gag polyprotein.

Roth H, Magg V, Uch F, Mutz P, Klein P, Haneke K, Lohmann V, Bartenschlager R, Fackler OT, Locker NS, Stoecklin G, Ruggieri A. (2017) 'Flavivirus infection uncouples translation suppression from cellular stress responses'. American Society for Microbiology mBio, Humoud MN, Doyle N, Royall E, Willcocks MM, Sorgeloos F, van Kuppeveld F, Roberts LO, Goodfellow IG, Langereis MA, Locker N. (2016) 'Feline Calicivirus Infection Disrupts Assembly of Cytoplasmic Stress Granules and Induces G3BP1 Cleavage'. AMER SOC MICROBIOLOGY JOURNAL OF VIROLOGY, 90 (14), pp. 6489-6501. Royall E, Locker N. (2016) 'Translational Control during Calicivirus Infection'. MDPI AG VIRUSES-BASEL, 8 (4) Article number ARTN 104 doi: 10.3390/v8040104 Royall E, Doyle N, Abdul-Wahab A, Emmott E, Morley SJ, Goodfellow I, Roberts LO, Locker N. (2015) 'Murine norovirus 1 (MNV1) replication induces translational control of the host by regulating eIF4E activity during infection.'. J Biol Chem, United States: 290 (8), pp. 4748-4758. doi: 10.1074/jbc.M114.602649