Protein structure, nuclear magnetic resonance spectroscopy, immunology, complement system

Use of time-resolved FRET to validate crystal structure of complement regulatory complex between C3b and factor H (N terminus), Pechtl IC, Neely RK, Dryden DT, Jones AC, Barlow PN, Protein Science 2011 [Epub ahead of print] PubMed PMID: 21936007. Estimation of interdomain flexibility of N-terminus of factor H using residual dipolar coupling, Maciejewski M, Tjandra N, Barlow PN, Biochemistry 2011 50 (38):8138-49. PubMed PMID: 21793561. Disease-associated N-terminal complement factor H mutations perturb cofactor and decay-accelerating activities, Pechtl IC, Kavanagh D, McIntosh N, Harris CL, Barlow PN, J Biol Chem 2011 286 (13):11082-90. PubMed PMID: 21270465. Structural basis for engagement by complement factor H of C3b on a self surface,Morgan HP, Schmidt CQ, Guariento M, Blaum BS, Gillespie D, Herbert AP, Kavanagh D, Mertens H, Svergun DI, Johansson CM, Uhrín D, Barlow PN, Hannan JP, Nature Struct Mol Biol 2011 18 (4):463-70. PubMed PMID: 21317894 Production of biologically active complement factor H in therapeutically useful quantities, Schmidt CQ, Slingsby FC, Richards A, Barlow PN, Protein Expr Purif 2011 76 (2):254-63. PubMed PMID: 21146613