Senior Lecturer in Pharmacology, University of East Anglia (2015 - present) Lecturer in Pharmacology, University of East Anglia (2013 - 2015) BBSRC David Phillips Fellow, University of East Anglia (2010 – 2013) BBSRC David Phillips Fellow, University of Leeds (2008 – 2010) Wellcome Trust Research Associate, University of Manchester (2004 – 2008) MRC-funded PhD, University of Leeds (2000 – 2004) BSc (Hons) Pharmacology, University of Leeds (1997 – 2000)

Our overarching research interests are the cellular and molecular mechanisms that underlie cardiovascular disease with an emphasis of artherosclerosis and vascular inflammation. We have a particular interest in signalling cues that generate calcium signals in leukocytes and how such signals regulate process of leukocyte migration and targeting, adhesion to vascular endothelium and secretion of pro- and anti-inflammatory cytokines. Examples of current projects include deciphering chemokine and nucleotide evoked calcium signalling in monocytes and their relationship to cardiovascular disease, and understanding how statins produce anti-inflammatory effects. We employ an array of cutting edge techniques including high-throughput calcium imaging, patch-clamp electrophysiology, time-lapse microscopy, virus production and virus-mediated gene delivery, primary blood cell isolation and compound screening to address these research questions.

Our research is generously funded by the British Heart Foundation and BBSRC.

Fountain, S., Sivaramakrishnan, V.(2015)Evidence for Extracellular ATP as a Stress Signal in a Single-Celled Organismin Eukaryotic Cell14.pp. 775-782 Full Text UEA Repository(Article) Campwala, H., Sexton, D. W., Crossman, D. C., Fountain, S. J.(2014)P2Y6 receptor inhibition perturbs CCL2-evoked signalling in human monocytic and peripheral blood mononuclear cellsin Journal of Cell Science127.pp. 4964-4973 Full Text UEA Repository(Article) Sivaramakrishnan, V., Fountain, S.(2013)Intracellular P2X receptors as novel calcium release channels and modulators of osmoregulation in Dictyostelium: A comparison of two common laboratory strainsin Channels7.pp. 43-46 Full Text UEA Repository(Article) Fountain, S. J.(2013)Primitive ATP-activated P2X receptors: discovery, function and pharmacologyin Frontiers in Cellular Neuroscience7. Full Text UEA Repository(Article) Campwala, H., Fountain, S. J.(2013)Constitutive and agonist stimulated ATP secretion in leukocytesin Communicative and Integrative Biology6.pp. e23631 Full Text UEA Repository(Article) Li, J., Fountain, S.(2012)Fluvastatin suppresses native and recombinant human P2X4 receptor functionin Purinergic Signalling8.pp. 311-316 Full Text UEA Repository(Article) Sivaramakrishnan, V., Fountain, S.(2012)A Mechanism of Intracellular P2X Receptor Activationin Journal of Biological Chemistry287.pp. 28315-28326 Full Text UEA Repository(Article) Sivaramakrishnan, V., Bidula, S., Campwala, H., Katikaneni, D., Fountain, S. J.(2012)Constitutive lysosome exocytosis releases ATP and engages P2Y receptors in human monocytesin Journal of Cell Science125.pp. 4567-4575 Full Text UEA Repository(Article) Fountain, S. J.(2010)Neurotransmitter Receptor Homologues of Dictyostelium discoideumin Journal of Molecular Neuroscience41.pp. 263-266 Full Text UEA Repository(Article) Cheong, A., Fountain, S. J., Beech, D. J.(2009)Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteriesin Potassium Channels491.pp. 19-33 Full Text UEA Repository(Article) Fountain, S. J., Burnstock, G.(2009)An evolutionary history of P2X receptorin Purinergic Signalling5.pp. 269-272 Full Text UEA Repository(Article) Young, M., Fisher, J., Fountain, S., Ford, R., North, A., Khakh, B.(2008)Molecular Shape, Architecture, and Size of Pin The Journal of Biological Chemistry283.pp. 26241-26251 Full Text UEA Repository(Article) Fountain, S., Cao, L., Young, M., North, A.(2008)Permeation Properties of a Pin The Journal of Biological Chemistry283.pp. 15122-15126 Full Text UEA Repository(Article) Cao, L., Young, M. T., Broomhead, H. E., Fountain, S. J., North, A. R.(2007)Thrin The Journal of Neuroscience27.pp. 12916-12923 Full Text UEA Repository(Article) Fountain, S. J., Cheong, A., Li, J., Dondas, N. Y., Zeng, F., Wood, I. C., Beech, D. J.(2007)Kin AJP - Heart and Circulatory Physiology293.pp. 2719-2725 Full Text UEA Repository(Article) Fountain, S. J., Parkinson, K., Young, M. T., Cao, L., Thompson, C. R. L., North, A. R.(2007)An intracellular P2X receptor required for osmoregulation in Dictyostelium discoideumin Nature448.pp. 200-203 Full Text UEA Repository(Article) Fountain, S. J., North, A. R.(2006)A C-terminal Lysine That Controls Human P2Xin The Journal of Biological Chemistry281.pp. 15044-15049 Full Text UEA Repository(Article) Zeng, F., Xu, S., Jackson, P. K., McHugh, D., Kumar, B., Fountain, S. J., Beech, D. R.(2004)Human TRPCin The Journal of Physiology559.pp. 739-750 Full Text UEA Repository(Article) Fountain, S. J., Cheong, A., Flemming, R., Mair, L., Sivaprasadarao, A., Beech, D. J.(2004)Functional up-regulation of KCNA gene family expression in murine mesenteric resistance artery smooth musclein The Journal of Physiology556.pp. 29-42 Full Text UEA Repository(Article)