Ph.D.: University of Colorado, Boulder 2000 Postdoc: University of Colorado Health Sciences Center

Cell Signaling Informatics & Proteomics Bioanalytical Chemistry

Yen, C.Y., Houel, S., Ahn, N.G., and Old, W.M. (2011) Spectrum-to-spectrum searching using a proteome-wide spectral library. Mol Cell Proteomics 10, M111 007666.

Meyer-Arendt, K., Old, W.M., Houel, S., Renganathan, K., Eichelberger, B., Resing, K.A., and Ahn, N.G. (2011) IsoformResolver: A Peptide-Centric Algorithm for Protein Inference. J Proteome Res 10, 3060-3075.

Houel, S., Abernathy, R., Renganathan, K., Meyer-Arendt, K., Ahn, N.G., and Old, W.M. (2010) Quantifying the impact of chimera MS/MS spectra on peptide identification in large-scale proteomics studies. J Proteome Res 9, 4152-4160.

Old, W.M., Shabb, J.B., Houel, S., Wang, H., Couts, K.L., Yen, C.-y., Litman, E.S., Croy, C.H., Meyer-Arendt, K., Miranda, J.G., Brown, R.A., Witze, E.S., Schweppe, R.E., Resing, K.A., and Ahn, N.G. (2009) Functional Proteomics Identifies Targets of Phosphorylation by B-Raf Signaling in Melanoma. Molecular Cell 34, 115-131.

Yen, C.Y., Meyer-Arendt, K., Eichelberger, B., Sun, S., Houel, S., Old, W.M., Knight, R., Ahn, N.G., Hunter, L.E., and Resing, K.A. (2009) A simulated MS/MS library for spectrum-to-spectrum searching in large-scale identification of proteins. Mol Cell Proteomics 8, 857-869.

Xu, Q., Zhu, S., Wang, W., Zhang, X., Old, W., Ahn, N., and Liu, X. (2009) Regulation of Kinetochore Recruitment of Two Essential Mitotic Spindle Checkpoint Proteins by Mps1 Phosphorylation. Mol. Biol. Cell 20, 10-20.

Wang, W., Yang, Y., Gao, Y., Xu, Q., Wang, F., Zhu, S., Old, W., Resing, K., Ahn, N., Lei, M., and Liu, X. (2009) Structural and mechanistic insights into Mps1 kinase activation. J Cell Mol Med 13, 1679-1694.

Holinger, E.P., Old, W.M., Giddings, T.H., Jr., Wong, C., Yates, J.R., 3rd, and Winey, M. (2009) Budding yeast centrosome duplication requires stabilization of Spc29 via Mps1-mediated phosphorylation. J Biol Chem 284, 12949-12955.

Xu, Q., Zhu, S., Wang, W., Zhang, X., Old, W., Ahn, N., and Liu, X. (2008) Regulation of Kinetochore Recruitment of Two Essential Mitotic Spindle Checkpoint Proteins by Mps1 Phosphorylation. Mol. Biol. Cell, E08-03-0324.

Witze, E.S., Old, W.M., Resing, K.A., and Ahn, N.G. (2007) Mapping protein post-translational modifications with mass spectrometry. Nat Methods 4, 798-806.

Sun, S., Meyer-Arendt, K., Eichelberger, B., Brown, R., Yen, C.Y., Old, W.M., Pierce, K., Cios, K.J., Ahn, N.G., and Resing, K.A.(2007) Improved validation of peptide MS/MS assignments using spectral intensity prediction. Mol Cell Proteomics 6, 1-17.

Mattison, C. P., Old, W.M., Steiner, E., Huneycutt, B. J., Resing, K. A., Ahn, N. G., and Winey, M. (2007) Mps1 activation loop autophosphorylation enhances kinase activity. J Biol Chem 282, 30553-30561.

Ahn, N.G., Shabb, J.B., Old, W.M., and Resing, K.A. (2007) Achieving in-depth proteomics profiling by mass spectrometry. ACS Chem Biol 2, 39-52.

Ruth, M.C., Old, W.M., Emrick, M.A., Meyer-Arendt, K., Aveline-Wolf, L.D., Pierce, K.G., Mendoza, A.M., Sevinsky, J.R., Hamady, M., Knight, R.D., Resing, K.A., and Ahn, N.G. (2006) Analysis of membrane proteins from human chronic myelogenous leukemia cells: comparison of extraction methods for multidimensional LC-MS/MS. J Proteome Res 5, 709-719.

Old, W.M., Meyer-Arendt, K., Aveline-Wolf, L., Pierce, K.G., Mendoza, A., Sevinsky, J. R., Resing, K.A., and Ahn, N.G. (2005) Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics. Mol Cell Proteomics 4, 1487-1502.

Russell, S.A., Old, W., Resing, K.A., and Hunter, L. (2004) Proteomic informatics. Int Rev Neurobiol 61, 127-157.

Resing, K.A., Meyer-Arendt, K., Mendoza, A.M., Aveline-Wolf, L.D., Jonscher, K.R., Pierce, K.G., Old, W.M., Cheung, H.T., Russell, S., Wattawa, J.L., Goehle, G.R., Knight, R.D., and Ahn, N.G. (2004) Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics. Analytical Chemistry 76, 3556-3568.