Cleary, Michael D. 照片

Cleary, Michael D.

Associate Professor

所属大学: University of California, Merced

所属学院: Molecular Cell Biology

邮箱:
mcleary4@ucmerced.edu

个人主页:
http://mcb.ucmerced.edu/content/michael-d-cleary

研究领域

I. Identification of mRNA decay networks in the nervous system We have developed a novel technique that allows measurement of mRNA decay in the nervous system of Drosophila. This technique provides the foundation for a systems-level approach that we are using to construct a neural development mRNA decay network comprised of the following information:genome-wide mRNA decay rates in neural cells of intact embryos, the cis-elements that target mRNAs for decay, the trans-acting RNA-binding proteins (RBPs) and microRNAs that recognize these cis-elements, and the spatial dynamics of mRNA-RBP interactions within neurons. Our long-term goal is to generate a comprehensive and predictive map of neural mRNA decay dynamics, thus filling a significant gap in current models of gene expression during neural development. II. Capturing physiological maps of neural gene expression Understanding brain function requires maps of neural connections and complementary measurements of neural activity. Gene expression is an essential component of neural activity: neurons change their gene expression in response to different stimuli and these changes have short-term and long-term effects on brain function. Identification of gene expression patterns in intact brains presents significant technical challenges. We are developing an improved RNA-tagging and purification technique (similar to TU-tagging) that will allow us to capture "snapshots" of gene expression in specific populations of neurons. Our current focus is on developing the necessary tools and applying this technique to the study of gene expression following ethanol exposure (in collaboration with Professor Fred Wolf at UC Merced). This work is supported by a grant from Cal-BRAIN.

近期论文

Burow DA, Umeh-Garcia MC, True MB, Bakhaj CD, Ardell DH, Cleary MD. Dynamic regulation of mRNA decay during neural development. Neural Development. 2015 Apr 21;10(1):11. Manansala MC, Min S, Cleary MD. The Drosophila SERTAD protein Taranis determines lineage-specific neural progenitor proliferation patterns. Developmental Biology. 2013. Apr 15;376(2):150-62. Gay L, Miller MR, Ventura PB, Devasthali V, Vue Z, Thompson HL, Temple S, Zong H, Cleary MD, Stankunas K, Doe CQ. Mouse TU tagging: a chemical/genetic intersectional method for purifying cell type-specific nascent RNA. Genes and Development. 2013. Jan. 1; 27: 98-115. Touma JJ, Weckerle FF, Cleary MD. Drosophila Polycomb complexes restrict neuroblast competence to generate motorneurons. Development. 2012 Feb; 139(4): 657-666. Miller MR, Robinson KJ, Cleary MD, Doe CQ. TU-tagging: cell type-specific RNA isolation from intact complex tissues. Nature Methods. 2009 May; 6: 439 – 441. Cleary MD and Doe CQ. Regulation of neuroblast competence: multiple temporal identity factors specify distinct neuronal fates within a single early competence window. Genes and Development. 2006 Feb; 20(4): 429-34. Cleary MD, Meiering CD, Jan E, Guymon R, and Boothroyd JC. Biosynthetic labeling of RNA via uracilphosphoribosyltransferase allows analysis of mRNA synthesis and decay by microarrays. Nature Biotechnology. 2005 Feb; 23(2): 232-7.