Our lab is interested in understanding the molecular mechanism of autophagy and membrane trafficking regulated by small GTPases by developing novel chemical and chemo-optogenetic approaches.

Modular photoswitchable molecular glues for chemo-optogenetic control of protein function in living cells Angewandte Chemie International Edition Zhang, Jun; Herzog, Laura K.; Corkery, Dale P.; et al. Visible-Light-Switchable molecular glues for reversible control of protein function Chemistry - A European Journal Zhang, Jun; Herzog, Laura K.; Li, Shuang; et al. ATG12–ATG5-TECPR1: an alternative E3-like complex utilized during the cellular response to lysosomal membrane damage Autophagy, Taylor & Francis 2024, Vol. 20, (2) : 443-444 Corkery, Dale P.; Wu, Yao-Wen A chemical inhibitor of IST1-CHMP1B interaction impairs endosomal recycling and induces noncanonical LC3 lipidation Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences 2024, Vol. 121, (17) Knyazeva, Anastasia; Li, Shuang; Corkery, Dale P.; et al. An ATG12-ATG5-TECPR1 E3-like complex regulates unconventional LC3 lipidation at damaged lysosomes EMBO Reports, EMBO Press 2023, Vol. 24, (9) Corkery, Dale; Castro-Gonzalez, Sergio; Knyazeva, Anastasia; et al. Eating while intoxicated: characterizing the molecular mechanism behind V. cholerae toxin MakA-regulated autophagy Autophagy, Taylor & Francis 2023, Vol. 19, (6) : 1885-1886 Corkery, Dale P.; Wu, Yao-Wen Inducin triggers LC3-lipidation and ESCRT-mediated lysosomal membrane repair ChemBioChem, Wiley-VCH Verlagsgesellschaft 2023, Vol. 24, (24) Corkery, Dale; Ursu, Andrei; Lucas, Belén; et al. Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles Nature Communications, Springer Nature 2023, Vol. 14, (1) Nanda, Suchet; Calderon, Abram; Sachan, Arya; et al. Controlling cellular activities with light Nature Methods, Nature Publishing Group 2023, Vol. 20 : 357-358 Wu, Yao-Wen V. cholerae MakA is a cholesterol-binding pore-forming toxin that induces non-canonical autophagy Journal of Cell Biology, Rockefeller University Press 2022, Vol. 221, (12) Jia, Xiaotong; Knyazeva, Anastasia; Zhang, Yu; et al. Monitoring the Response of Multiple Signal Network Components to Acute Chemo-Optogenetic Perturbations in Living Cellsope ChemBioChem, John Wiley & Sons 2022, Vol. 23, (4) Kowalczyk, Manuela; Kamps, Dominic; Wu, Yao-Wen; et al. Synthesis of 20-Membered Macrocyclic Pseudo-Natural Products Yields Inducers of LC3 Lipidation Angewandte Chemie International Edition, Wiley-VCH Verlagsgesellschaft 2022, Vol. 61, (11) Niggemeyer, Georg; Knyazeva, Anastasia; Gasper, Raphael; et al. Ultrafast and selective labeling of endogenous proteins using affinity-based benzotriazole chemistry Chemical Science, Royal Society of Chemistry 2022, Vol. 13, (24) : 7240-7246 Xin, Xiaoyi; Zhang, Yu; Gaetani, Massimiliano; et al. Thermal proteome profiling identifies the membrane-bound purinergic receptor P2X4 as a target of the autophagy inhibitor indophagolin Cell Chemical Biology, Elsevier 2021, Vol. 28, (12) : 1750-1757.e5 Carnero Corrales, Marjorie A.; Zinken, Sarah; Konstantinidis, Georgios; et al. Vibrio cholerae cytotoxin MakA induces noncanonical autophagy resulting in the spatial inhibition of canonical autophagy Journal of Cell Science, The Company of Biologists 2021, Vol. 134, (5) Corkery, Dale; Nadeem, Aftab; Aung, Kyaw Min; et al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) Autophagy, Taylor & Francis 2021, Vol. 17, (1) : 1-382 Corkery, Dale; Wu, Yao-Wen; Dowaidar, Moataz Monitoring the Response of Multiple Signal Network Components to Acute Chemo-Optogenetic Perturbations in Living Cellsope ChemBioChem (Print) Kowalczyk, Manuela; Kamps, Dominic; Wu, Yao-Wen; et al. Imaging of spatial cycling of Rab GTPase in the cell Rab GTPases: methods and protocols, Humana Press 2021 : 105-115 Li, Fu; Wu, Yao-Wen RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein Autophagy, Taylor & Francis 2021, Vol. 17, (9) : 2290-2304 Pantoom, Supansa; Konstantinidis, Georgios; Voss, Stephanie; et al. Spatiotemporal Imaging of Small GTPase Activity Using Conformational Sensors for GTPase Activity (COSGA) Ras Activity and Signaling: Methods and Protocols, Humana Press 2021 : 259-267 Wu, Yao-Wen Phenotyping Reveals Targets of a Pseudo-Natural-Product Autophagy Inhibitor Angewandte Chemie International Edition, Wiley-VCH Verlagsgesellschaft 2020, Vol. 59, (30) Foley, Daniel J.; Zinken, Sarah; Corkery, Dale; et al. Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network Cell reports, Cell Press 2020, Vol. 33, (9) Kamps, Dominic; Koch, Johannes; Juma, Victor O.; et al. Image-Based Morphological Profiling Identifies a Lysosomotropic, Iron-Sequestering Autophagy Inhibitor Angewandte Chemie International Edition, Wiley-VCH Verlagsgesellschaft 2020, Vol. 59 : 5721-5729 Laraia, Luca; Garivet, Guillaume; Foley, Daniel J.; et al. Inhibition of K-Ras4B-plasma membrane association with a membrane microdomain-targeting peptide Chemical Science, Royal Society of Chemistry 2020, Vol. 11, (3) : 826-832 Li, Fang-Yi; Zhang, Zhen-Feng; Voss, Stephanie; et al. Distinct Mechanisms for Processing Autophagy Protein LC3-PE by RavZ and ATG4B ChemBioChem (Print), Wiley-VCH Verlagsgesellschaft 2020, Vol. 21, (23) : 3377-3382 Yang, Aimin; Pantoom, Supansa; Wu, Yao-Wen