1997–2001 Department of Industrial Chemistry, Faculty of Engineering, Kyoto University 2001–2003 Department of Molecular Engineering, Graduate School of Engineering, Kyoto University 2003–2007 Department of Structural Molecular Science, School of Physical Sciences, The Graduate University for Advanced Studies 2007–2009 Postdoctoral Researcher, JST-ICORP, Institute of Frontier Medical Sciences, Kyoto University 2009– Project Assistant Professor, Department of Chemistry, School of Science, The University of Tokyo Awards: 2012 CSJ Presentation Award 2006 CSJ Student Presentation Award

Biomolecular science/Bioimaging

Advances in fluorescence and bioluminescence imaging T. Ozawa, H. Yoshimura and S.B. Kim, Anal. Chem.,85, 590-609 (2013). Fluorescent probes for imaging endogenous β-actin mRNA in living cells using fluorescent protein-tagged pumilio. H. Yoshimura, A. Inaguma, T. Yamada and T. Ozawa ACS Chem. Biol., 7, 999-1005 (2012). Visualization of non-engineered single mRNAs in living cells using genetically encoded fluorescent probes. T. Yamada, H. Yoshimura, A. Inaguma and T. Ozawa, Anal. Chem., 83, 5708-5714 (2011). Hydrogen bonding interaction on the heme-bound ligand in the heme-based O2 sensor protein, M. Nishimura, H. Yoshimura, K. Ozawa, S. Yoshioka, M. Kubo, T. Kitagawa and S. Aono, J. Porphyrins Phthalocyanines, 12, 142-148 (2008). Protein conformation changes of HemAT-Bs upon ligand binding probed by ultraviolet resonance Raman spectroscopy, S. F. EI-Mashtoly, Y. Gu, H. Yoshimura, S. Yoshioka, S. Aono, T. Kitagawa, J. Biol. Chem., 283, 6942-6946 (2008). The signal transduction mechanism of HemAT-Bs through the proximal heme pocket revealed by time-resolved resonance Raman spectroscopy, H. Yoshimura, S. Yoshioka, Y. Mizutani and S. Aono, Biochem. Biophys. Res. Commun., 307, 1053-1057 (2007). Two ligand binding sites in the O2-sensing signal transducer HemAT: implications for ligand recognition/discrimination and signaling, E. Pinakoulaki, H. Yoshimura, V. Daskalakis, S. Yoshioka, S. Aono and C. Varotsis, Proc. Natl. Acad. Sci. USA, 103, 14796-14801 (2006). Specific hydrogen-bonding networks responsible for selective O2 sensing of the oxygen sensor protein HemAT from Bacillus subtilis, H. Yoshimura, S. Yoshioka, K. Kobayashi, T. Ohta, T. Uchida, M. Kubo, T. Kitagawa and S. Aono, Biochemistry, 45, 8301-8307 (2006). Recognition and discrimination of gases by the oxygen-sensing signal transducer protein HemAT as revealed by FTIR spectroscopy, E. Pinakoulaki, H. Yoshimura, S. Yoshioka, S. Aono and C. Varotsis, Biochemistry, 45, 7763-7766 (2006). Non-covalent modification of the heme-pocket of apomyoglobin by a 1,10-phenanthroline derivative, Y. Hitomi, H. Mukai, H. Yoshimura, T. Tanaka and T. Funabiki, Bioorg. Med. Chem. Lett., 16, 248-251 (2006). Biophysical properties of a c-type heme in chemotaxis signal transducer protein DcrA, S. Yoshioka, K. Kobayashi, H. Yoshimura, T. Uchida, T. Kitagawa and S. Aono, Biochemistry, 44, 15406.-15413 (2005). Oxygen-sensing mechanism of HemAT from Bacillus subtilis: a resonance Raman spectroscopic study, T. Ohta, H. Yoshimura, S. Yoshioka, S. Aono and Teizo Kitagawa,

J. Am. Chem. Soc., 126, 15000-15001 (2004).