B.S. Chemistry, Biology, & French, Purdue University (2002); Ph.D. Chemistry, The Scripps Research Institute (2008); NIH Postdoctoral Fellow, California Institute of Technology (2009-2012)

Biochemistry & Chemical Biology

Biocatalysis, Chemical Biology, Protein Engineering, Directed Evolution, Unnatural Amino Acid Mutagenesis, X-ray crystallography

Structure-Guided Directed Evolution of Highly selective P450-Based Magnetic Resonance Imaging Sensors for Dopamine and Serotonin. E.M. Brustad, V.S. Lelyveld, C.D. Snow, N. Crook, S.T. Jung, F.M. Martinez, T.J. Scholl, A. Jasanoff, F.H. Arnold. J. Mol. Biol. (2012) article in press DOI:10.1016/j.jmb.2012.05.029 Piecewise Protein Structure Determination. E.M. Brustad, P.A. Romero, F.H. Arnold, C.D. Snow. manuscript in preparation Structure-Guided Engineering of Lactococcus lactis Alcohol Dehydrogenase LlAdhA to Produce. Isobutanol. X. Liu, S. Bastian, C.D. Snow, E.M. Brustad, T. Saleski, P. Meinhold, F.H. Arnold. manuscript submitted (2012) Metal-Substituted Protein MRI Contrast Agents Engineered for Enhanced Relaxivity and Ligand Sensitivity. V.S. Lelyveld, E.M. Brustad, F.H. Arnold, A. Jasanoff. J. Am. Chem. Soc. (2011) 133(4), 649 - Optimizing Non-natural Protein Function with Directed Evolution. E.M. Brustad, F.H. Arnold. Curr. Opin. Chem. Biol. (2011) 15(2), 201 - 210 Localization of GroEL Determined by in vivo Incorporation of a Fluorescent Amino Acid. G. Charbon, J. Wang, E. Brustad, P.G. Schultz, A.L. Horwich, C. Jacobs-Wagner, E. Chapman. Bioorg. Med. Chem. Lett. (2011) 21(20), 6067-6070 Subcellular Protein Localization by Using a Genetically Encoded Fluorescent Amino Acid. G. Charbon, E. Brustad, K.A. Scott, J. Wang, A. Lobner-Oelsen, P.G. Shultz, C. Jacobs-Wagner, E. Chapman. Chembiochem (2011) 12(12), 1818 - 1821 Genetically Encoded Alkenes in Yeast. H.W. Ai, W. Shen, E. Brustad, P.G. Schultz. Angew. Chem. Int. Ed. (2010) 49(5), 935 – 937 Site-Directed Spin Labeling of a Genetically Encoded Unnatural Amino Acid. M. Fleissner*, E.M. Brustad*, T. Kalai, C. Altenbach, D. Cascio, F.B. Peters, K. Hideg, S. Peuker, P.G. Schultz, W.L. Hubbell. Proc. Natl. Acad. Sci. U.S.A (2009) 106(51), 21637 - 21642 (*Authors contributed equally) Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability. E.A. Lemke, Y. Gambin, V. Vandelinder, E.M. Brustad, H.W. Liu, P.G. Schultz, A. Groisman, A.A. Deniz. J. Am. Chem. Soc. (2009) 131(38), 13610 - 13612 Evolution of Proteins with Genetically Encoded ‘Chemical Warheads’. C.C. Liu, A.V. Mack, E.M. Brustad, J.H. Mills, D. Groff, V.V. Smyder, P.G. Schultz. J. Am. Chem. Soc. (2009) 131(28), 9616 - 9617 A General and Efficient Method for the Site-Specific Dual Labeling of Proteins for Single Molecule FRET. E.M. Brustad, E.A. Lemke, P.G. Schultz, A.A. Deniz. J. Am. Chem. Soc. (2008) 130(52), 17664 -17665 A Promiscuous Aminoacyl-tRNA Synthetase that Incorporates Cysteine, Methionine, and Alanine Homologs into Proteins. E. Brustad, M.L. Bushey, A. Brock, J. Chittiluru, P.G. Schultz. Bioorg. Med. Chem. Lett. (2008) 18(22), 6004 - 6006 A Genetically Encoded Boronate-Containing Amino Acid. E. Brustad, M.L. Bushey, J.W. Lee, D. Groff, W. Liu, P.G. Schultz. Angew. Chem. Int. Ed. (2008) 47(43), 8220 - 8223 Crystal Structure of a Biosynthetic Sulfo-hirudin Complexed to Thrombin. C.C. Liu, E. Brustad, W. Liu, P.G. Schultz. J. Am. Chem. Soc. (2007) 129(35), 10648 -10649

The Incorporation of a Photoisomerizable Amino Acid into Proteins in E. coli. M. Bose, D. Groff, J. Xie, E. Brustad, P. G. Schultz. J. Am. Chem. Soc. (2006) 128(2), 388 - 389