Dr Sean Hudson is an ARC Discovery Early Career Researcher Award (DECRA) Fellow at the RSC, ANU. He completed his B.Sc. Honours (Chemistry) in 2008 at the University of Adelaide with Prof. John Carver, working on small-molecule inhibitors of amyloid fibril formation. Thereafter, he undertook his Ph.D.(Chemistry) with Prof. Chris Abell at the University of Cambridge, researching in fragment-based drug discovery against the Tuberculosis (TB) cytochrome P450 (CYP) enzymes. Prior to arriving at ANU, Sean has been working at the University of California in San Francisco (UCSF), researching proximity-labelling technologies for protein interactomics, and in applications of tethered fragment-based ligand discovery.

Misfolded protein interactions

Proteomics & Molecular Biology Proximity-labelling enzymes Protein-protein interaction proteomics Expression vector construction Mammalian cell/tissue culture and transfection Affinity purification and peptide mass spectrometry Bacterial recombinant protein expression and purification PAGE/Western blotting Cell staining, imaging and viability analysis Biophysics, Biochemistry & Structural Biology X-ray crystallography Isothermal titration calorimetry Absorbance, fluorescence and CD spectroscopy Nuclear magnetic resonance spectroscopy Transmission electron microscopy Dynamic light scattering Thermal shift Plate readers Enzyme kinetics Mass spectrometry Organic Chemistry, Drug Discovery & Pharmacology Organic synthesis Compound characterization Fragment/compound library assembly and screening Peptide synthesis Cheminformatics & Biostatistics Physicochemical property determination Chemical diversity analysis Molecular fingerprinting and similarity QM energy calculations Chemical database management and searching Statistical significance tests