M.S., Pharmacy, Medical Academy, 1981 Ph.D., Analytical Chemistry, Medical Academy, 1989 Postdoctoral Fellow, University d'Aix Marseille, 1990-1991

Metabolomics is a fascinating new field for understanding of biological systems and discovering new biomarkers. A minimally modified silica hydride stationary phase operating in the ANP (Aqueous Normal Phase) mode with MS compatible mobile phases is an appropriate material for the retention and separation of hydrophilic compounds typically found in metabolic samples. Coupled with detection by mass spectrometry, the analysis of multicomponent mixtures of hydrophilic compounds can be accomplished. The Diamond Hydride stationary phase used in ANP mode for separation of metabolites was developed at SJSU in Prof. Pesek's group. It is now commercialized.

“A New Approach to Bioanalysis: Aqueous Normal Phase Chromatography with Silica Hydride Stationary Phases,” J.J. Pesek, M.T. Matyska, Bioanalysis, 4 (2012) 845-853. “Analysis of cycloserine and related compounds using aqueous normal phase chromatography/mass spectrometry”, J.J. Pesek, M.T. Matyska, A. Dang, J. Pharm. Biomed. Anal., 64-65 (2012) 72-76. “Determination of accessible silanols groups on silica gel surfaces using microcalorimetric measurements”, B. Buszewski, S. Bocian, G. Rychlicki, M. Matyska, J. Pesek, J. Chromatogr. A 1232 (2012) 43-46. “Simultaneous separation of hydrophobic and hydrophilic peptides with a silica hydride stationary phase using aqueous normal phase conditions”, R.I. Boysen, Y. Yang, J. Chowdhury, M.T. Matyska, J.J. Pesek, M.T.W. Hearn, J. Chromatogr. A,. 1218 (2011) 8021-8026. “Improvement of Peak Shape in Aqueous Normal Phase Analysis of Anionic Metabolites", J.J. Pesek, M.T. Matyska, S.M. Fischer, J. Sep. Sci., 34 (2011) 3509-3516.