B.S., Chemistry, Peking University Ph.D, Chemistry, The Pennsylvania State University Scientist, SurroMed, Inc. (2000-2002) Senior Scientist, Nanoplex Technologies, Inc. (2002-2003)

Bioanalytical Chemistry

Significant advances in modern technology have given us opportunities to explore and comprehend nature at a level of detail never dreamed of. To continue this fast-paced progress, the development of novel strategic methodologies to address complex scientific questions is imperative. Equipped with interdisciplinary approaches, our research focuses on developing novel analytical tools and sensors and exploiting their applications in the field of bioanalysis. In particular, we are interested in coupling the novel properties of various nanomaterials with conventional analytical techniques to develop:

1) amplification-by-polymerization in portable DNA diagnostic kit development;

2) label-free multiplexed biomolecule detection using encoded nanoparticles;

3) plasmon-enhanced single particle imaging of anisotropic nanoparticles; and

4) nanofeatures-facilitated mass spectrometric techniques in biomedical imaging and metabolite profiling.

Neupane, B.; Chase, T. E.; Zhao, L.; He, L.*; Wang, G*. "Enhanced Radiative Properties of Metallic Junctions in Segmented Au-Ag Nanowires", J. Phys. Chem. C, 2014, submitted. Moening, T. N.; Brown V. L.; He, L.* "Nanostructure-Initiator Mass Spectrometry (NIMS) for Molecular Mapping of Animal Tissues" book chapter in "Mass Spectrometric Imaging of Small Molecules", Methods in Molecular Biology Series, He. L, Eds. Humana Press 2014 in print. Brown V. L.; Liu, Q.; He, L.* "Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) for Mass Spectrometry Imaging of Small Molecules " book chapter in "Mass Spectrometric Imaging of Small Molecules", Methods in Molecular Biology Series, He. L, Eds. Humana Press 2014 in print. Liu, Q.; Miyata, H.; He, L.* "Mesoporous TiOx Substrates for Surface-Assisted Laser Desorption Ionization Mass Spectrometry Imaging", Rapid Comm. Mass Spec. 2014, in preparation. Zheng, W.; He, L.* "Thermodynamic and Kinetic Study of Formation of Cationic Conjugated Polymer-DNA Complexes", Bioscience Mater. 2014, accepted. Zheng, W.; Chase, T. E.; He, L.* "Label-free Multiplexing Detection of miRNA Using Conjugated Polymers and Striped Nanoparticles", Anal Method, 2014, 6, 2399-2405. Liu, Q.; Brown, V. L.; He, L.* "Desorption/Ionization on Porous Silicon (DIOS) for Metabolite Imaging" Invited chapter for inclusion in "Porous Silicon for Biomedical Applications", Woodhead Publishing Series in Biomaterials 68#, H. A. Santos, Eds. Woodhead Publishing 2013. pg 270-285. Zheng, W.; He, L.* "Multiplexed Immunoassays" Invited chapter in Multiplexed Immunoassays, Advances in Immunoassay Technology, Norman H. L. Chiu (Ed.), InTech, 2012. He, P.; Tucker, E. Z.; Gorman, C. B.; He, L.* "Chemical Amplification for In-Gel DNA Detection" Anal. Method. 2011, 3, 2463-2468. Wu Y.; Liu S.; He, L. "Polymerization-Assisted Signal Amplification for Electrochemical Detection of Biomarkers" Analyst 2011, 136, 2558-2563. Wu, Y.; Liu, S.; He, L. "Activators Generated Electron Transfer for Atom Transfer Radical Polymerization for Immunosensing", Biosensor Bioelectron. 2010, 26, 970-975. Zheng, W.; He, L.* "Distance-Dependent Fluorescence Quenching of Conjugated Polymer on Au/Ag Striped Nanorods", J. Phys. Chem. C, 2010, 114, 17829–17835. Qian, H. He, L.*"Polymeric Macroinitiators for Signal Amplification in AGET ATRP-Based DNA Detection", Sens. Actuator B, 2010, 150, 594-600. Liu, Q.; Xiao, Y.; He, L.* "Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS)" Invited chapter for inclusion in "Mass Spectrometric Imaging: History, Fundamentals and Protocols", Methods in Molecular Biology Series, Jonathan Sweedler and Stanislav Rubakhin, Eds. Humana Press. Zheng, W.; He, L.* "Multiplexed Detection of Protein Cancer Markers on Au/Ag-Barcoded Nanorods Using Fluorescent-Conjugated Polymers" Anal. Bioanal. 2010, 397, 2261–2270. Qian, H.; He, L.* "Detection of Protein Binding Using Polymerization-Assisted Amplification" Anal Chem. 2009, 81, 9824–9827. Liu, Q.; He, L.* "Ionic Matrix for Matrix-Enhanced Surface-Assisted Laser Desorption Ionization Imaging Mass Spectrometry (ME-SALDI-IMS)" J. Am. Soc. Mass Spectrom. 2009, 20, 2229–2237. Wu, Y.; Liu, S.; He, L.* "Electrochemical Biosensing Using Amplification-by-Polymerization", Anal. Chem. 2009, 81, 7015–7021. Qian, H. He, L.* "Surface-initiated AGET ATRP for Detection of DNA Point Mutation" Anal Chem. 2009, 81, 4536–4542. He, P.; He, L.* "Synthesis of Surface-Anchored DNA-Polymer Bioconjugates Using Reversible Addition-Fragmentation Chain Transfer Polymerization (RAFT)", Biomacromolecules, 2009, 10, 1804–1809.

Zheng, W.; He, L.* "Label-Free, Real-Time Multiplexed DNA Detection Using Fluorescent Conjugated Polymers" J. Am. Chem. Soc. 2009, 131, 3432–3433.