Our research encompasses two main areas - enzymes, and molecular imaging probes. You can find out more about our work under the Research tab.

Research

Transition State Analysis

Enzyme Mechanisms and Inhibition

Imaging Infections

Lou, M., Gilpin, M. E., Burger, S. K., Malik, A. M., Gawuga, V., Popovic, V., Capretta, A. & Berti, P. J. (2012) J. Am. Chem. Soc. 134:12947-12957 Transition State Analysis of Acid-Catalyzed Hydrolysis of an Enol Ether, Enolpyruvylshikimate 3-Phosphate (EPSP) (link)

Lou, M., Burger, S. K., Gilpin, M. E., Gawuga, V., Capretta, A. & Berti, P. J. (2012) J. Am. Chem. Soc. 134: 12958-12969 Transition State Analysis of Enolpyruvylshikimate 3-Phosphate Synthase (AroA)-Catalyzed EPSP Hydrolysis (link)

Rob, T., Liuni, P., Gill, P. K., Zhu, S., Balachandran, N., Berti, P. J. & Wilson, D. J. (2012) Anal. Chem. 84: 3771-3779 Measuring dynamics in weakly structured regions of proteins using microfluidics-enabled subsecond H/D exchange mass spectrometry (link)

Jiang, S., Gilpin, M. E., Attia, M., Ting, Y.-L. & Berti, P. J. (2011) Biochemistry 50: 2205-2212 Lyme Disease Enolpyruvyl-UDP-GlcNAc Synthase: Fosfomycin-Resistant MurA from Borrelia burgdorferi, a Fosfomycin-Sensitive Mutant, and the Catalytic Role of the Active Site Asp (link)

Jackson, S. G., Zhang, F., Chindemi, P., Junop, M. S. & Berti, P. J. (2009) Biochemistry 48:11715-11723 Evidence for Kinetic Control of Ligand Binding and Staged Product Release in MurA (Enolpyruvyl UDP-GlcNAc Synthase)-Catalyzed Reactions (link)

Berti, P. J. & Chindemi, P. (2009) Biochemistry 48:3699-3707 Catalytic Residues and an Electrostatic Sandwich That Promote Enolpyruvyl Shikimate 3-Phosphate Synthase (AroA) Catalysis (link)

McCann, J. A. B. & Berti, P. J. (2008) J. Am. Chem. Soc. 130:5789-5797 Transition State Analysis of the DNA Repair Enzyme MutY (link)

McCann, J. A. B. & Berti, P. J. (2007) J. Am. Chem. Soc. 129:7055-7064 Transition State Analysis of Acid-catalyzed dAMP Hydrolysis (link)

Clark, M. E. & Berti, P. J. (2007) Biochemistry 46:1933-1940 Enolpyruvyl Activation by Enolpyruvyl Shikimate 3-Phosphate Synthase (link)

Zhang, F. & Berti, P. J. (2006) Biochemistry 45:6027-6037 Phosphate Analogues as Probes of the Catalytic Mechanisms of MurA and AroA, Two Carboxyvinyl Transferases (link)

Loncke, P. G. & Berti, P. J. (2006) J. Am. Chem. Soc. 128:6132-6140 Implications of Protonation and Substituent Effects for C-O and O-P Bond Cleavage in Phosphate Monoesters (link)

Berti, P. J. & McCann, J. A. B. (2006) Chem. Rev. 106:506-555 Toward a Detailed Understanding of Base Excision Repair Enzymes: Transition State and Mechanistic Analyses of N-Glycoside Hydrolysis and N-Glycoside Transfer (link)

Mizyed, S., Oddone, A., Byczynski, B., Hughes, D. W., & Berti, P. J. (2005) Biochemistry 44:4011-4017 UDP-N-acetylmuramic acid (UDP-MurNAc) is a Potent Inhibitor of MurA (Enolpyruvyl-UDP-GlcNAc Synthase) (link)

Lee, J. K., Bain, A. D. & Berti, P. J. (2004) J. Am. Chem. Soc. 126:3769-3776 Probing the Transition States of Four Glucoside Hydrolyses with 13C Kinetic Isotope Effects Measured at Natural Abundance by NMR Spectroscopy. (link)

Byczynski, B., Mizyed, S. & Berti, P. J. (2003) J. Am. Chem. Soc. 125:12541-12550 Non-enzymatic Breakdown of the Tetrahedral (α-Carboxyketal Phosphate) Intermediates of MurA and AroA, Two Carboxyvinyl Transferases. Protonation of Different Functional Groups Control the Rate and Fate of Breakdown. (link)

McCann, J. A. B. & Berti, P. J. (2003) J. Biol. Chem. 278:29587-29592 Adenine Release is Fast in MutY-Catalyzed Hydrolysis of G:A and 8-oxo-G:A DNA Mismatches (link)

Mizyed, S., Wright, J. E. I., Byczynski, B. & Berti, P. J. (2003) Biochemistry 42:6986-6995 Identification of the Catalytic Residues of AroA (Enolpyruvylshikimate 3-Phosphate Synthase) Using Partitioning Analysis (link)

Berti, P. J., & Tanaka, K. S. E. (2002) Adv. Phys. Org. Chem. 37:239-314 Transition State Analysis Using Multiple Kinetic Isotope Effects: Mechanisms of Enzymatic and Non-enzymatic Glycoside Hydrolysis and Transfer (link)

Chen, X.-Y., Berti, P. J. & Schramm, V. L. (2000) J. Am. Chem. Soc. 122:6527-6534 Transition State Analysis for Depurination of DNA by Ricin A-Chain

Chen, X.-Y., Berti, P. J. & Schramm, V. L. (2000) J. Am. Chem. Soc. 122:1609-1617 Ricin A-Chain: Kinetic Isotope Effects and Transition State Structure with Stem-Loop RNA