Mitochondria play a central role in basic physiological functions by producing most of the cellular ATP. Furthermore, mitochondria are important for cellular functions such as heat production, generation of reactive oxygen species (ros) and execution of the apoptotic program. Given the key role of mitochondria in various cellular events, it is not surprising that mitochondrial dysfunctions are seen in many pathological conditions such as neurodegeneration, diabetes, aging and cancer. The aim of my research is to understand the regulation of mitochondrial metabolism under physiological and pathological conditions using unbiased screening approaches, notably metabolic profiling in combination with global gene expression analysis.