Dr Alex Ivanov is Senior Lecturer (Assoc. Prof.) at the Department of Chemistry. His research focuses on the innovation of novel nanoscale sensors and platforms for single-molecule detection and delivery, that enable the studying of fundamentally important molecules in chemistry, biology, and medicine. Examples include tunnelling nanopore platform for high-resolution DNA fragment sizing and sequencing Nano Letters (2011), ACS Nano (2014), Nature Communications (2021A), Science Advances (2022), optical/plasmonic nanopore platforms Nano Letters (2013, 2015); ACS Nano (2015A), methods for on-demand delivery of single molecules ACS Nano (2015B), single-molecule detection in ultra-dilute (fM) samples Nature Communications (2016), nanopore/field-effect transistors for selective bioanalyte sensing Nature Communications (2017A), zeptolitre confinement of single molecules Nano Letters (2017, 2018), multiplexed single-molecule protein detection in human serum, Nature Communications (2017B, 2019,2021B), Nature Nanotechnology (2023), single-molecule nanotweezers that can extract single DNA, proteins and organelles from living cells, Nature Nanotechnology (2019) to name a few.

Nanoelectronics Single-molecule and single-cell methods Nanopore platforms, single-molecule delivery, and detection (nucleic acids and proteins) Single-molecule biomarker detection (cancer, neurodegeneration, cardiovascular disease, ...) Point-of-care diagnostics

Ren R, Cai S, Fang X, Wang X, Zhang Z, Damiani M, Hudlerova C, Rosa A, Hope J, Cook NJ, Gorelkin P, Erofeev A, Novak P, Badhan A, Crone M, Freemont P, Taylor GP, Tang L, Edwards C, Shevchuk A, Cherepanov P, Luo Z, Tan W, Korchev Y, Ivanov AP, Edel JBclose, 2023, Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes, Nature Communications, Vol: 14, ISSN: 2041-1723 Kwan Z, Paulose Nadappuram B, Leung MM, Mohagaonkar S, Li A, Amaradasa KS, Chen J, Rothery S, Kibreab I, Fu J, Sanchez-Alonso JL, Mansfield CA, Subramanian H, Kondrashov A, Wright PT, Swiatlowska P, Nikolaev VO, Wojciak-Stothard B, Ivanov AP, Edel JB, Gorelik Jclose, 2023, Microtubule-Mediated Regulation of β2AR Translation and Function in Failing Hearts., Circ Res, Vol: 133, Pages: 944-958 Liu Y, Wang X, Campolo G, Teng X, Ying L, Edel JB, Ivanov APclose, 2023, Single-Molecule Detection of α-Synuclein Oligomers in Parkinson's Disease Patients Using Nanopores., ACS Nano Koch C, Reilly-ODonnell B, Gutierrez R, Lucarelli C, Ng FS, Gorelik J, Ivanov AP, Edel JBclose, 2023, Nanopore sequencing of DNA-barcoded probes for highly multiplexed detection of microRNA, proteins and small biomarkers, Nature Nanotechnology, ISSN: 1748-3387 Jiang T, Yi L, Liu X, Ivanov AP, Edel JB, Tang Lclose, 2023, Fabrication of electron tunneling probes for measuring single-protein conductance, Nature Protocols, Vol: 18, Pages: 2579-2599, ISSN: 1750-2799 Koch C, Reilley-O'Donnell B, Gutierrez R, Lucarelli C, Ng FS, Gorelik J, Ivanov AP, Edel JBclose, 2023, Novel screening platform for highly multiplexed biomarker detection, Publisher: SPRINGER, Pages: S190-S190, ISSN: 0175-7571 Sahota A, Monteza Cabrejos A, Kwan Z, Paulose Nadappuram B, Ivanov AP, Edel JBclose, 2023, Recent advances in single-cell subcellular sampling., Chemical Communications, Vol: 59, Pages: 5312-5328, ISSN: 1359-7345 Wang X, Thomas T-M, Ren R, Zhou Y, Zhang P, Li J, Cai S, Liu K, Ivanov AP, Herrmann A, Edel JBclose, 2023, Nanopore detection using supercharged polypeptide molecular carriers, Journal of the American Chemical Society, Vol: 145, Pages: 6371-6382, ISSN: 0002-7863 Fried JP, Swett JL, Nadappuram BP, Fedosyuk A, Gee A, Dyck OE, Yates JR, Ivanov AP, Edel JB, Mol JAclose, 2022, Localised solid-state nanopore fabrication via controlled breakdown using on-chip electrodes, Nano Research, Vol: 15, Pages: 9881-9889, ISSN: 1998-0124 Tang L, Yi L, Jiang T, Ren R, Paulose Nadappuram B, Zhang B, Wu J, Liu X, Lindsay S, Ivanov A, Edel Jclose, 2022, Measuring conductance switching in single proteins using quantum tunneling, Science Advances, Vol: 8, Pages: 1-8, ISSN: 2375-2548 Ren R, Sun M, Goel P, Cai S, Kotov NA, Kuang H, Xu C, Ivanov A, Edel JBclose, 2021, Single-molecule binding assay using nanopores and dimeric NP conjugates, Advanced Materials, Vol: 33, ISSN: 0935-9648 Fried JP, Swett JL, Nadappuram BP, Fedosyuk A, Sousa PM, Briggs DP, Ivanov AP, Edel JB, Mol JA, Yates JRclose, 2021, Understanding electrical conduction and nanopore formation during controlled breakdown, Small, Vol: 17, Pages: 1-9, ISSN: 1613-6810 Oh S-H, Altug H, Jin X, Low T, Koester SJ, Ivanov AP, Edel JB, Avouris P, Strano MSclose, 2021, Nanophotonic biosensors harnessing van der Waals materials, NATURE COMMUNICATIONS, Vol: 12, ISSN: 2041-1723 Al Sulaiman D, Gatehouse A, Ivanov AP, Edel JB, Ladame Sclose, 2021, Length-Dependent, Single-Molecule Analysis of Short Double-Stranded DNA Fragments through Hydrogel-Filled Nanopores: A Potential Tool for Size Profiling Cell-Free DNA, ACS APPLIED MATERIALS & INTERFACES, Vol: 13, Pages: 26673-26681, ISSN: 1944-8244 Cai S, Pataillot-Meakin T, Shibakawa A, Ren R, Bevan C, Ladame S, Ivanov A, Edel Jclose, 2021, Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum, Nature Communications, Vol: 12, ISSN: 2041-1723 Ying Y-L, Ivanov AP, Tabard-Cossa V, 2021, No small matter, NATURE CHEMISTRY, Vol: 13, Pages: 216-217, ISSN: 1755-4330 Fried JP, Swett JL, Nadappuram BP, Mol JA, Edel JB, Ivanov AP, Yates JRclose, 2021, In situ solid-state nanopore fabrication., Chemical Society Reviews, ISSN: 0306-0012 Tang L, Paulose Nadappuram B, Cadinu P, Zhao Z, Xue L, Yi L, Ren R, Wang J, Ivanov A, Edel Jclose, 2021, Combined quantum tunnelling and dielectrophoretic trapping for molecular analysis at ultra-low analyte concentrations, Nature Communications, Vol: 12, Pages: 1-8, ISSN: 2041-1723 Ren R, Wang X, Cai S, Korchev Y, Ivanov A, Edel Jclose, 2020, Selective sensing of proteins using aptamer functionalised nanopore extended field-effect transistors, Small Methods, Vol: 4, Pages: 1-8, ISSN: 2366-9608 Xue L, Yamazaki H, Ren R, Wanunu M, Ivanov AP, Edel JBclose, 2020, Solid-state nanopore sensors (Sep, 10.1038/s41578-020-0229-6, 2020), NATURE REVIEWS MATERIALS, Vol: 5, Pages: 952-952, ISSN: 2058-8437 Xue L, Yamazaki H, Ren R, Wanunu M, Ivanov AP, Edel JBclose, 2020, Solid-state nanopore sensors, Nature Reviews Materials, Vol: 5, Pages: 931-951, ISSN: 2058-8437 Wang X, Wilkinson MD, Lin X, Ren R, Willison KR, Ivanov AP, Baum J, Edel JBclose, 2020, Correction: Single-molecule nanopore sensing of actin dynamics and drug binding, Chemical Science, Vol: 11, Pages: 8036-8038, ISSN: 2041-6520 Clark R, Nawawi MA, Dobre A, Pugh D, Liu Q, Ivanov AP, White AJP, Edel JB, Kuimova MK, McIntosh AJS, Welton Tclose, 2020, The effect of structural heterogeneity upon the microviscosity of ionic liquids, Chemical Science, Vol: 11, Pages: 6121-6133, ISSN: 2041-6520 Cadinu P, Kang M, Paulose Nadappuram B, Ivanov A, Edel JBet al., 2020, Individually addressable multi-nanopores for single-molecule targeted operations, Nano Letters, Vol: 20, Pages: 2012-2019, ISSN: 1530-6984 Wang X, Wilkinson MD, Lin X, Ren R, Willison KR, Ivanov A, Baum J, Edel Jclose, 2020, Single-molecule nanopore sensing of actin dynamics and drug binding, Chemical Science, Vol: 11, Pages: 970-979, ISSN: 2041-6520 Zhang Y, Takahashi Y, Hong SP, Liu F, Bednarska J, Goff P, Novak P, Shevchuk A, Gopal S, Barozzi I, Magnani L, Sakai H, Suguru Y, Fujii T, Gorelkin P, Majouga A, Weiss D, Edwards C, Ivanov A, Klenerman D, Sviderskaya E, Edel J, Korchev Yclose, 2019, High-resolution label-free 3D mapping of extracellular pH of single living cells, Nature Communications, Vol: 10, Pages: 1-9, ISSN: 2041-1723 Wang X, Wilkinson MD, Lin X, Ren R, Willison K, Ivanov AP, Baum J, Edel JBclose, 2019, Single-molecule nanopore sensing of actin dynamics and drug binding Cai S, Sze JYY, Ivanov AP, Edel JBclose, 2019, Small molecule electro-optical binding assay using nanopores., Nat Commun, Vol: 10 Paulose Nadappuram B, Cadinu P, Barik A, Ainscough A, Devine M, Kang M, Gonzalez-Garcia J, Kittler J, Willison K, Vilar Compte R, Actis P, Wojciak-Stothard B, Oh S-H, Ivanov A, Edel JBclose, 2019, Nanoscale tweezers for single-cell biopsies, Nature Nanotechnology, Vol: 14, Pages: 80-88, ISSN: 1748-3387 Xue L, Cadinu P, Paulose Nadappuram B, Kang M, Ma Y, Korchev Y, Ivanov A, Edel Jclose, 2018, Gated single-molecule transport in double-barreled nanopores, ACS Applied Materials and Interfaces, Vol: 10, Pages: 38621-38629, ISSN: 1944-8244 Single-molecule methods have been rapidly developing with the appealing prospect of transforming conventional ensemble-averaged analytical techniques. However, challenges remain especially in improving detection sensitivity and controlling molecular transport. In this article, we present a direct method for the fabrication of analytical sensors that combine the advantages of nanopores and field-effect transistors for simultaneous label-free single-molecule detection and manipulation. We show that these hybrid sensors have perfectly aligned nanopores and field-effect transistor components making it possible to detect molecular events with up to near 100% synchronization. Furthermore, we show that the transport across the nanopore can be voltage-gated to switch on/off translocations in real time. Finally, surface functionalization of the gate electrode can also be used to fine tune transport properties enabling more active control over the translocation velocity and capture rates.Abstract Open Access Link Cite Al Sulaiman D, Cadinu P, Ivanov AP, Edel JB, Ladame Sclose, 2018, Chemically modified hydrogel-filled nanopores: a tunable platform for single-molecule sensing, Nano Letters: a journal dedicated to nanoscience and nanotechnology, Vol: 18, Pages: 6084-6093, ISSN: 1530-6984 Ivanov AP, Edel JB, 2018, Scissoring genes with light, NATURE CHEMISTRY, Vol: 10, Pages: 800-801, ISSN: 1755-4330 Cadinu P, Campolo G, Pud S, Yang W, Edel J, Dekker C, Ivanov Aclose, 2018, Double barrel nanopores as a new tool for controlling single-molecule transport, Nano Letters, Vol: 18, Pages: 2738-2745, ISSN: 1530-6984 Peveler WJ, Noimark S, Al-Azawi H, Hwang GB, Crick CR, Allan E, Edel JB, Ivanov AP, MacRobert AJ, Parkin IPclose, 2018, Covalently attached antimicrobial surfaces using BODIPY: improving efficiency and effectiveness, ACS Applied Materials and Interfaces, Vol: 10, Pages: 98-104, ISSN: 1944-8244 Kunstmann-Olsen C, 2018, Joshua Edel, Aleksandar Ivanov, and MinJun Kim (Eds): Nanofluidics, 2nd Edn, Chromatographia, Vol: 81, Pages: 173-173, ISSN: 0009-5893 Sze JYY, Ivanov AP, Cass AEG, Edel JBclose, 2017, Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers, Nature Communications, Vol: 8, Pages: 1-10, ISSN: 2041-1723 Crick CR, Albella P, Kim H-J, Ivanov AP, Kim K-B, Maier SA, Edel JBclose, 2017, Low-Noise Plasmonic Nanopore Biosensors for Single Molecule Detection at Elevated Temperatures, ACS Photonics, Vol: 4, Pages: 2835-2842, ISSN: 2330-4022 Ren R, Zhang Y, Paulose Nadappuram B, Akpinar B, Klenerman D, Ivanov AP, Edel JB, Korchev Yclose, 2017, Nanopore extended field effect transistor for selective single molecule biosensing, Nature Communications, Vol: 8, Pages: 1-9, ISSN: 2041-1723 Cadinu P, Paulose Nadappuram B, Lee DJ, Sze JYY, Campolo G, Zhang Y, Shevchuk A, Albrecht T, Korchev Y, Ivanov AP, Edel JBclose, 2017, Single molecule trapping and sensing using dual nanopores separated by a zeptoliter nanobridge, Nano Letters, Vol: 17, Pages: 6376-6384, ISSN: 1530-6984 Lin X, Ivanov AP, Edel JB, 2017, Selective single molecule nanopore sensing of proteins using DNA aptamer-functionalised gold nanoparticles, Chemical Science, Vol: 8, Pages: 3905-3912, ISSN: 2041-6539 Kim M, Ivanov A, Edel J, 2017, Preface, ISBN: 9781849734042 Pitchford WH, Crick CR, Kim H-J, Ivanov AP, Kim H-M, Yu J-S, Albrecht T, Kim K-B, Edel JBclose, 2017, Low Noise Nanopore Platforms Optimised for the Synchronised Optical and Electrical Detection of Biomolecules, NANOFLUIDICS, 2ND EDITION, Editors: Edel, Ivanov, Kim, Publisher: ROYAL SOC CHEMISTRY, Pages: 270-300, ISBN: 978-1-84973-404-2 Edel J, Ivanov A, Kim M, 2016, Nanofluidics (Second Edition), Publisher: Royal Society of Chemistry, ISBN: 9781849734042 Crick CR, Noimark S, Peveler WJ, Bear JC, Ivanov AP, Edel JB, Parkin IPclose, 2016, Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging, Jove-Journal of Visualized Experiments, Vol: 113, ISSN: 1940-087X Freedman KJ, Crick CR, Albella P, Barik A, Ivanov AP, Maier SA, Oh SH, Edel JBclose, 2016, On-Demand Surface and Tip Enhanced Raman Spectroscopy Using Dielectrophoretic Trapping and Nanopore Sensing, ACS Photonics, Vol: 3, Pages: 1036-1044, ISSN: 2330-4022 Edel JB, Freedman K, Otto L, Ivanov A, Barik A, Oh Sclose, 2016, Nanopore sensing at ultra-low concentrations using single molecule dielectrophoretic trapping, Nature Communications, Vol: 7, Pages: 1-9, ISSN: 2041-1723 Turek VA, Francescato Y, Cadinu P, Crick CR, Elliott L, Chen Y, Urland V, Ivanov AP, Hong M, Vilar R, Maier SA, Giannini V, Edel JBclose, 2015, Self-Assembled Spherical Supercluster Metamaterials from Nanoscale Building Blocks, ACS Photonics, Vol: 3, Pages: 35-42, ISSN: 2330-4022 Daeubler M, Ivanov A, Sjenitzer BL, Sanchez V, Stieglitz R, Macian-Juan Rclose, 2015, High-fidelity coupled Monte Carlo neutron transport and thermal-hydraulic simulations using Serpent 2/SUBCHANFLOW, Annals of Nuclear Energy, Vol: 83, Pages: 352-375, ISSN: 0306-4549 Ivanov A, Sanchez V, Stieglitz R, Ivanov Kclose, 2015, Large-scale Monte Carlo neutron transport calculations with thermal hydraulic feedback, Annals of Nuclear Energy, Vol: 84, Pages: 204-219, ISSN: 0306-4549 Crick CR, Noimark S, Peveler WJ, Bear JC, Ivanov AP, Edel J, Parkin IPclose, 2015, Advanced analysis of nanoparticle composites – A neans toward increasing the efficiency of functional materials, RSC Advances, Vol: 5, Pages: 53789-53795, ISSN: 2046-2069 Sze JYY, Kumar S, Ivanov AP, Oh SH, Edel JBclose, 2015, Fine tuning of nanopipettes using atomic layer deposition for single molecule sensing, Analyst, Vol: 14, Pages: 4828-4834, ISSN: 0003-2654 Ivanov AP, Actis P, Jönsson P, Klenerman DK, Korchev Y, Edel JBclose, 2015, On-demand delivery of single DNA molecules using nanopipettes, ACS Nano, Vol: 9, Pages: 3587-3595, ISSN: 1936-086X Pitchford WH, Kim H-J, Ivanov AP, Kim H-M, Yu J-S, Leatherbarrow RJ, Albrecht T, Kim K-B, Edel JBclose, 2015, Synchronized Optical and Electronic Detection of Biomolecules Using a Low Noise Nanopore Platform, ACS NANO, Vol: 9, Pages: 1740-1748, ISSN: 1936-0851 Ivanov A, Sanchez V, 2015, Variance reduction in high resolution coupled Monte Carlo-thermal-hydraulics calculations, Pages: 1981-1994 Sanchez V, Ivanov A, Hoogenboom JE, 2015, Towards the development of coupled Monte Carlo/subchannel thermal hydraulic codes for high-fidelity simulation of LWR full cores, Pages: 1968-1980 Hoogenboom JE, Ivanov A, Sanchez V, 2015, Maximum efficiency in massively parallel execution of Monte Carlo criticality calculations, Pages: 1436-1448 Crick CR, Albella P, Ng B, Roschuk T, Ivanov AP, Cecchini MP, Bresme F, Maier SA, Edel JBclose, 2014, Precise Attolitre Temperature Control of Nanopore Sensors using a Nanoplasmonic Bullseye, Nano Letters, Vol: 15, Pages: 553-559, ISSN: 1530-6992 Ivanov A, Sanchez V, Stieglitz R, Ivanov Kclose, 2014, Internal multi-scale multi-physics coupled system for high fidelity simulation of light water reactors, Annals of Nuclear Energy, Vol: 66, Pages: 104-112, ISSN: 0306-4549 Ivanov AP, Freedman KJ, Kim MJ, Albrecht T, Edel JBclose, 2014, High Precision Fabrication and Positioning of Nanoelectrodes in a Nanopore, ACS Nano