Staff Scientist, R&D, Stratagene, La Jolla, CA Ph.D. Utah State University, Logan, UT B.S. California State University Chico, Chico, CA

Dr. Clark’s research interests span a broad array of topics in biochemical education.

Broberg CA, Clark DD (2010) Shotgun proteomics of Xanthobacter autotrophicus Py2 reveals proteins specific to growth on propylene. Archives of Microbiology 192: 945-957. Boyd JM, Clark DD, Kofoed MA, Ensign SA (2010) Mechanism of inhibition of aliphatic epoxide carboxylation by the coenzyme M analog 2-bromoethanesulfonate. The Journal of Biological Chemistry 285: 25232-25242. Krishnakumar AM, Nocek BP, Clark DD, Ensign SA, Peters JW (2006) Structural basis for stereoselectivity in the (R)- and (S)-hydroxypropylthioethanesulfonate dehydrogenases. Biochemistry 45:8831-8840. Pandey AS, Nocek B, Clark DD, Ensign SA, Peters JW (2006) Mechanistic implications of the structure of the mixed-disulfide intermediate of the disulfide oxidoreductase, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase. Biochemistry 45:113-120. Clark DD, Boyd JM, Ensign SA (2004) The Stereoselectivity and catalytic properties of Xanthobacter autotrophicus 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase are controlled by interactions between C-terminal arginine residues and the sulfonate of coenzyme M. Biochemistry 43: 6763-6771. Nocek B, Jang SB, Jeong MS, Clark DD, Ensign SA, Peters JW (2002) Structural basis for CO2-fixation by a novel member of the disulfide oxidoreductase family of enzymes, 2-ketopropyl-coenzyme oxidoreductase/carboxylase. Biochemistry 41: 12907-12913. Nocek B, Clark DD, Ensign SA, Peters JW (2002) Crystallization and preliminary X-ray analysis of an (R)-2-hydroxypropyl-coenzyme M dehydrogenase. Acta Crystallographica 58: 1470-1473. Clark DD, Ensign SA (2002) Characterization of the 2-[(R)-2 hydroxypropylthio]-ethanesulfonate dehydrogenase from Xanthobacter strain Py2: product inhibition, pH dependence of kinetic parameters, site-directed mutagenesis, rapid equilibrium inhibition, and chemical modification. Biochemistry 41:2727-2740. Jang SB, Jeong MS, Clark DD, Ensign SA, Peters JW (2001) Crystallization and preliminary X-ray analysis of a NADPH: 2-ketopropyl-coenzyme M oxidoreductase/carboxylase. Acta Crystallographica 57: 445-447. Clark DD, Allen JR, Ensign SA (2000) Characterization of five catalytic activities associated with the NADPH 2-(2-ketopropylthioethane sulfonate) oxidoreductase carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system. Biochemistry 39: 1294 -1304 Allen JR, Clark DD, Krum JG, Ensign SA (1999) A role for coenzyme M (2- mercaptoethanesulfonate) in a bacterial pathway of aliphatic epoxide carboxylation. Proceedings of the National Academy of Sciences USA 96: 8432-8437 Clark DD, Ensign SA (1999) Evidence for an inducible nucleotide dependent acetone carboxylase in Rhodococcus rhodochrous strain B-276. The Journal of Bacteriology 181: 2752-2758.