Use of the fission yeast Schizosaccharomyces pombe for chemical genetic study of cyclic nucleotide phosphodiesterases (PDEs) and other cAMP pathway components from mammals and pathogens.

Fantes P.A. and Hoffman C.S. (2016). A Brief History of Schizosaccharomyces pombe Research: A Perspective Over the Past 70 Years. Genetics, 203:621-9. doi: 10.1534/genetics.116.189407 Takemata N, Oda A, Yamada T, Galipon J, Miyoshi T, Suzuki Y, Sugano S, Hoffman CS, Hirota K, Ohta K. (2016). Local potentiation of stress-responsive genes by upstream noncoding transcription. Nucleic Acids Res. 44:5174-89. doi: 10.1093/nar/gkw142 Xu C, Wyman AR, Alaamery MA, Argueta SA, Ivey FD, Meyers JA, Lerner A, Burdo TH, Connolly T, Hoffman CS, Chiles TC. (2016). Anti-inflammatory effects of novel barbituric acid derivatives in T lymphocytes. Int Immunopharmacol. 2016 Sep;38:223-32. doi: 10.1016/j.intimp.2016.06.004 Hoffman, C.S., Wood, V. and Fantes P.A (2015) An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System Genetics. 2015 Oct;201(2):403-23. doi: 10.1534/genetics.115.181503. de Medeiros A.S. and Hoffman C.S. (2015) A yeast-based high-throughput screen for modulators of phosphodiesterase activity. Methods Mol Biol. 2015;1294:181-90. doi: 10.1007/978-1-4939-2537-7_14. de Medeiros A.S., Kwak G., Vanderhooft J., Rivera S., Gottlieb R., and Hoffman C.S. (2015) Fission Yeast-Based High-Throughput Screens for PKA Pathway Inhibitors and Activators. Methods Mol Biol.1263:77-91. doi: 10.1007/978-1-4939-2269-7_6. Asada R, Takemata N, Hoffman CS, Ohta K, Hirota K. (2014) Antagonistic controls of chromatin and mRNA start site selection by Tup family corepressors and the CCAAT-binding factor Mol Cell Biol. pii: MCB.00924-14. [Epub ahead of print] PMID:25535331 Mudge D.K., Yang F., Currie B.M., Kim J.M., Yeda K., Bashyakarla V.K., Ivey F.D., and Hoffman C.S. (2014). Sck1 negatively-regulates Gpa2-mediated glucose signaling in Schizosaccharomyces pombe. Eukaryot Cell. 2014 Feb;13(2):202-8. doi: 10.1128/EC.00277-13. Demirbas, D., Wyman, A.R., Shimizu-Albergine, M., Cakici, O., Beavo, J.A., and Hoffman, C.S. (2013). A Yeast-Based High-Throughput Screen Identifies A Phosphodiesterase Inhibitor That Elevates Steroidogenesis In Mouse Leydig Cells Via PDE8 And PDE4 Inhibition. PLoS One. 2013;8(8):e71279. doi: 10.1371/journal.pone.0071279. de Medeiros, A.S., Magee, A., Nelson, K., Friedberg, L., Trocka, K., and Hoffman, C.S. (2013). Use of PKA-mediated phenotypes for genetic and small molecule screens in Schizosaccharomyces pombe. Biochem Soc Trans. 2013 Dec 1;41(6):1692-5. doi: 10.1042/BST20130159. Ceyhan O., Birsoy K., and Hoffman C.S. 2012. Identification of biologically active PDE11-selective inhibitors using a yeast-based high throughput screen, Chemistry & Biology, 19:155-163. Mudge D.K., Hoffman C.A., Lubinski T.J., and Hoffman C.S. 2012. Use of a ura5+-lys7+ cassette to construct unmarked gene knock-ins in Schizosaccharomyces pombe, Current Genetics, 58: 59-64. Didem Demirbas, Ozge Ceyhan, Arlene R. Wyman and Charles S. Hoffman 2011. A Fission Yeast-based Platform for Phosphodiesterase Inhibitor HTSs and Analyses of Phosphodiesterase Activity, Handbook of Experimental Pharmacology (Houslay, Francis, and Conti eds.) Phosphodiesterases as Drug Targets 2011 (204): 135-149. Demirbas, D., Ceyhan, O., Wyman, A.R., Ivey, F.D., Allain, C., Wang, L., Sharuk, M.N., Francis, S.H., and Hoffman, C.S. 2011. Use of a Schizosaccharomyces pombe PKA-repressible reporter to study cGMP-metabolising phosphodiesterases. Cellular Signalling. 23(3):594-601. PubMed Ivey, F.D., Taglia, F.X., Yang, F., Lander, M.M., Kelly, D.A., and Hoffman, C.S. 2010. Activated alleles of the Schizosaccharomyces pombe gpa2+ Gα gene identify residues involved in GDP-GTP exchange. Eukaryotic Cell 9: 626–633. PubMed Alaamery, M.A., Wyman, A.R., Ivey, F.D., Allain, C., Demirbas, D., Wang, L., Ceyhan, O., and Hoffman, C.S. 2010. New classes of PDE7 inhibitors identified by a fission yeast-based HTS. Journal of Biomolecular Screening 15:359–367. PubMed Roux, A.E., Leroux, A., Alaamery, M.A., Hoffman, C.S., Chartrand, P., Ferbeyre, G., and Rokeach, L.A. 2009. Pro-aging effects of glucose signaling through a G protein-coupled glucose receptor in fission yeast. PLoS Genetics 5(3): e1000408. PubMed Hirota, K., Miyoshi, T., Kugou, K., Hoffman, C.S., Shibata, T., and Ohta, K. 2008. Stepwise chromatin remodelling by a cascade of transcription initiation of non-coding RNAs. Nature 456: 130–134. PubMed Leem, Y.-E., Ripmaster, T., Kelly, F., Ebina, H., Heincelman, M., Zhang, K., Grewal, S.I.S., Hoffman, C. S., and Levin, H. L. 2008. The pol II promoters of Schizosaccharomyces pombe are targeted by an LTR retrotransposon that is capable of repairing the promoters it disrupts. Molecular Cell 30: 98–107. PubMed Alaamery, M.A., and Hoffman, C.S. 2008. Schizosaccharomyces pombe Hsp90/Git10 is required for glucose/cAMP signaling. Genetics 178: 1927–36. Ivey, F.D., Wang, L., Demirbas, D., Allain, C., Hoffman, C.S. 2008. Development of a fission yeast-based high-throughput screen to identify chemical regulators of cAMP phosphodiesterases. Journal of Biomolecular Screening 13: 62–71. PubMed Hoffman, C.S. 2007. Propping up our knowledge of G protein signaling pathways: diverse functions of putative noncanonical Gbeta subunits in fungi. Science's STKE 370: 3. PubMed Kao, R.S., Morreale, E., Wang, L., Ivey, F.D., Hoffman, C.S. 2006. Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase. Genetics 173: 49–61. PubMed Hoffman, R.L., and Hoffman, C.S. 2006. Cloning the Schizosaccharomyces pombe lys2+ gene and construction of new molecular genetic tools. Current Genetics 49: 414–420. PubMedWang, L., Griffiths, K., Zhang, Y.H., Ivey, F.D., Hoffman, C.S. 2005. Schizosaccharomyces pombe adenylate cyclase suppressor mutations suggest a role for cAMP phosphodiesterase regulation in feedback control of glucose/cAMP signaling. Genetics 171: 1523–1533. PubMed