黄磊，男，1977年10月出生，博士，副教授，博士生导师 浙江大学，化工与生物工程学院，生物工程研究所。 E-mail lhuangblue@zju.edu.cn 地址 杭州市浙江大学紫金港校区和同苑化1-407 教育经历 2006年9月，浙江大学生物化工工学博士；2000年6月，浙江大学化学工程学士学位。 工作经历 12/2011 – 现在 浙江大学 化学工程与生物工程学系 生物工程研究所 副教授 06/2009 – 12/2011 浙江大学 化学工程与生物工程学系 生物工程研究所 讲师 02/2007 – 05/2009 新加坡南洋理工大学 化学与生物分子工程系 博士后

生物化工

1）微生物转录调控和基因组重排新技术； 2）重要临床和工业用酶的分子改造； 3）合成生物学指导下的基因组进化及其生物产品工程。

[1]. X Zhang, JY Sheng, L Huang*, LP Du, J Cai, PL Cen, ZN Xu*. (2014) High-level soluble expression of one model olfactory receptor (ODR-10) in Escherichia coli cell-free system. World J. Microbiol. Biotechnol., 30(3): 893-901. [2].JY Sheng, L Huang*, XC Zhu, J Cai, ZN Xu*. (2014) Reconstitution of the peptidoglycan cytoplasmic precursor biosynthetic pathway in cell-free system and rapid screening of antisense oligonucleotides for Mur enzymes. Appl. Microbiol. Biotechnol., 98(4): 1785-1794. [3].X Zhang, JZ Lian, L Kai, L Huang*, PL Cen, ZN Xu*. (2014) Enhanced functional expression of aquaporin Z via fusion of in situ cleavable leader peptides in Escherichia coli cell-free system. Enzyme Microb. Technol., 55: 26-30. [4].HQ Chong#, L Huang#, JW Yeow, Ivy Wang, HF Zhang, H Song, RR Jiang*. (2013) Improving Ethanol Tolerance of Escherichia coli by Rewiring Its Global Regulator cAMP Receptor Protein (CRP), PLoS One, 8(2):e57628. [5].F Zhu, J Cai, XT Wu, J Huang, L Huang*, JZ Zhu, Q Zheng, PL Cen, ZN Xu*. (2013) The main byproducts and metabolic flux profiling of gamma-PGA-producing strain B. subtilis ZJU-7 under different pH values. J Biotechnol. 164(1): 67-74. [6].W Luo, J Huang, XC Zhu, L Huang, J Cai, ZN Xu*. (2013) Enhanced Production of L-Tryptophan with Glucose Feeding and Surfactant Addition and Related Metabolic Flux Redistribution in the Recombinant Escherichia coli. Food Sci. Biotechnol. 22(1): 207-214. [7].J Huang, S Jiang, XQ Xu, HS Wu, XC Zhu, ZM Ke, J Cai, L Huang, ZN Xu*.(2012). Effects of carbon/nitrogen ratio, dissolved oxygen and impeller type on gellan gum production in Sphingomonas paucimobilis. Ann. Microbiol. 62(1): 299-305. [8].L Huang, YJ Xiang, J Cai, L Jiang, ZB Lv, YZ Zhang, ZN Xu*. (2011) Effects of three main sugars in cane molasses on the production of butyric acid with Clostridium tyrobutyricum. Korean J. Chem. Eng. 28(12): 2312-2315. [9].Z Yang, RR Ma, L Huang, XC Zhu, JY Sheng, J Cai, W Hu, ZN Xu*.(2012) High-level production of soluble adenine nucleotide translocator from Schistosoma japonicum in E. coli cell-free system. Process Biochem. 47(3):395-400. [10].WM Gao, XC Zhu, J Cai, L Huang, PL Cen, ZN Xu*.(2012) Refolding and two-step purification by hydrophobic interaction chromatography of recombinant human bone morphogenetic protein-2 from Escherichia coli. Process Biochem. 47(6):960-967. [11].YP Liu, JF Pan, PL Wei, JZ Zhu, L Huang, J Cai*, ZN Xu*.(2012) Efficient expression and purification of recombinant alcohol oxidase in Pichia pastoris. Biotechnol. Bioprocess Eng. 17(4):693-702. [12].XC Zhu, Q Zheng, H Yang, J Cai1, L Huang, YW Duan,ZN Xu*, PL Cen.(2012) Recent advances in inkjet dispensing technologies: applications in drug discovery. Expert Opin. Drug Discov.. 7(9):761-770. [13].Y Yu, PL Wei, XC Zhu*, L Huang, J Cai, ZN Xu*.(2012) High-level production of soluble pyrroloquinoline quinone -dependent glucose dehydrogenase in Escherichia coli. Eng. Life Sci. 12(5):1-9. [14].W Luo, J Cai*, LQ Zhu, XC Zhu, L Huang, ZN Xu*, PL Cen.(2012) Toxic effects of acrylic acid on Clostridium propionicum and isolation of acrylic acid-tolerant mutants for potential production of acrylic acid. Eng. Life Sci. 12(5):567-573. [15].ZM Shi, PL Wei, XC Zhu, J Cai, L Huang, ZN Xu*.(2012) Efficient production of L-lactic acid from hydrolysate of Jerusalem artichoke with immobilized cells of Lactococcus lactis in fibrous bed bioreactors. Enzyme Microb. Technol. 51(5):263-268. [16].HL Zhang, JZ Zhu, XC Zhu, J Cai, AY Zhang, YZ Hong, J Huang, L Huang, ZN Xu*: High-level exogenous glutamic acid-independent production of poly-(c-glutamic acid) with organic acid addition in a new isolated Bacillus subtilis C10. Bioresour Technol. 116: 241-246. [17].Q Zheng, R Shi, XC Zhu, L Huang, J Cai, WB Han, ZN Xu*.(2012) Functional expression of Bacillus subtilis xylanase A in an Escherichia coli derived cell-free protein synthesis system and subsequent expression improvement via DNA gel technique. Process Biochem. 116: 241-246. [18].LQ Zhu, PL Wei, J Cai, XC Zhu, ZM Wang, L Huang, ZN Xu*.(2012) Improving the productivity of propionic acid with FBB-immobilized cells of an adapted acid-tolerant Propionibacterium acidipropionici. Bioresour Technol. 11(3):238-244. [19].J Huang, J Cai, J Wang, XC Zhu, L Huang, ST Yang, ZN Xu*.(2011) Efficient production of butyric acid from Jerusalem artichoke by immobilized Clostridium tyrobutyricum in a fibrous-bed bioreactor. Bioresour Technol., 108(1):31-40. [20].Q Zheng, JG Lu, H Chen, L Huang, J Cai, ZN Xu*. (2011) Application of inkjet printing technique for biological material delivery and antimicrobial assays. Anal Biochem. 410(2): 171-178.