管文军，女，浙江大学医学院教授/博导，现任浙江大学药物生物技术研究所副所长、浙江省微生物生化与代谢工程重点实验室副主任。担任中国微生物学会理事，浙江省微生物学会常务理事兼秘书长。 主要研究领域为微生物合成生物学和微生物制药。近年来主持和参与了国家重点研发计划、国家863计划、国家自然科学基金、浙江省自然科学基金重点项目、浙江省科技计划重点项目等多项国家级和省部级课题，获授权国家发明专利6项，发表SCI/EI论文40余篇，获得省部级一等奖、二等奖3项。在PLoS Genet, mBio, Mol Microbiol, Genome Res, Appl Environ Microbiol , Microb Cell Factories, FMES Yeast Res, Biosens Bioelectron等国际主流杂志上发表了多篇论文。 学习工作经历 2001.3-2004.6 浙江大学生物医学工程与仪器学学院 博士 2006.11-2008.10 美国康奈尔大学 访问学者 1993.9-2005.12 浙江大学生命科学学院 助教、讲师 2005.12-2013.12 浙江大学生命科学学院 副教授 2013.12-2015.12 浙江大学生命科学学院 教授 2015.12- 浙江大学药物生物技术研究所 教授 2016.7- 浙江大学药物生物技术研究所 副所长 2015.4- 浙江省微生物生化与代谢工程重点实验室 副主任 研究方向 1. 高效微生物底盘细胞设计 基于合成生物学理念，通过对微生物细胞代谢途径的设计和优化，构建基于链霉菌、酵母的底盘细胞，异源高效合成小分子药物和表达医药用蛋白。 2.微生物耐药机制 多药耐药转运蛋白的结构-功能、转运网络的应答调控机制研究 ；细菌毒力因子的发现及其致病机制研究。 欢迎有生物制药研究兴趣，有生物学、化学专业背景的学生报考本实验室研究生！ 诚邀优秀的博士毕业生到本实验室进行博士后研究！待遇从优！ 奖励荣誉 [1] 浙江省科学技术奖二等奖，复合酶制剂在羊毛织物绿色后整理中的应用，2008.12 (排名3) [2] 浙江省科学技术奖一等奖，生物反应与分离耦合技术在轻工医药中的应用，2008.12 (排名6) [3] 教育部科技进步奖一等奖，酶法合成与分离耦合技术在有机酸产品开发中的应用，2009.12(排名6) [4] 中国商业联合会科技进步奖二等奖，羊毛织物生物后整理技术的应用开发，2009.12(排名6)

微生物合成生物学 微生物耐药机制 微生物制药

Selected publications Jianxin Dong, Jiali Ning, Yu Tian, Han Li, Hua Chen, Wenjun Guan*. The involvement of multiple ABC transporters in daunorubicin efflux in Streptomyces coeruleorubidus. Microbial Biotechnology. 2024; 17: e70023 Quanchao Zhu, Zuyuan Jia,Yuchao Song, Weiwang Dou, Daniel Henry Scharf, Xiaodan Wu, Zhihao Xu*, Wenjun Guan*. Effects of PpSpi1, a GPI-anchored cell wall glycoprotein on cell wall defects of N-glycosylation engineered Pichia pastoris. mBio. 2023.14 (5): 00617-23. Jiaxiu Wei, Binbin Chen, Jianxin Dong, Xueyu Wang, Yongquan Li, Yingchun Liu*, Wenjun Guan*. Salinomycin biosynthesis reversely regulates the β-oxidation pathway in Streptomyces albus by carrying a 3-hydroxyacyl-CoA dehydrogenase gene in its biosynthetic gene cluster. Microbial Biotechnology. 2022;15:2890–2904. Jianxin Dong, Jiaxiu Wei, Han Li, Shiyao Zhao, Wenjun Guan*. An efficient markerless deletion system suitable for the industrial strains of Streptomyces. Journal of Microbiology and Biotechnology. 2021, 31(12): 1722-1731. Weiwang Dou#, Quanchao Zhu#, Meihua Zhang, Zuyuan Jia, Wenjun Guan*. Screening and evaluation of the strong endogenous promoters in Pichia pastoris. Microbial Cell Factories. 2021, 20:156. Shiyao Zhao#, Dong Guo#, Quanchao Zhu, Weiwang Dou, Wenjun Guan*. Display of Microbial Glucose Dehydrogenase and Cholesterol Oxidase on the Yeast Cell Surface for the Detection of Blood Biochemical Parameters. Biosensors. 2021, 11(1): 13. Han Li, Jiaxiu Wei, Jianxin Dong, Yudong Li, Yongquan Li, Yinghu Chen, Wenjun Guan*. Enhanced Triacylglycerol Metabolism Contributes to Efficient Oil Utilization and High Production of Salinomycin in Streptomyces albus ZD11. Applied and Environmental Microbiology. 2020, 86(16): e00763-20. Yiming Shan, Dong Gou, Quanshu Gu, Yudong Li, Yongquan Li, Yinghu Chen*, Wenjun Guan*. Genome Mining and homologous comparison strategy for digging Exporters contributing Self-resistance in Natamycin-producing Streptomyces strains. Applied Microbiology and Biotechnology. 2020, 104: 817-831. Tanjun Wang, Yiming Shan, Han Li, Weiwang Dou, Xinhang Jiang, Xuming Mao, Shuiping Liu, Wenjun Guan*, Yongquan Li*, Multiple transporters are involved in natamycin efflux in Streptomyces chattanoogensis L10. Molecular Microbiology. 2017, 103(4): 713–728. Zhenhong Zhu,Han Li, Pin Yu, Yuanyang Guo, Shuai Luo,Zhongbin Chen, Xuming Mao, Wenjun Guan, Yongquan Li*. SlnR is a positive pathway-specific regulator for salinomycin biosynthesis in Streptomyces albus. Applied Microbiology and Biotechnology. 2017, 101:1547–1557. Weiwang Dou, Jianhua Zhu, Tanjun Wang, Wei Wang, Han Li, Xin Chen, Wenjun Guan*. Mutations of charged amino acids at the cytoplasmic end of transmembrane helix 2 affect transport activity of the budding yeast multidrug resistance protein Pdr5p. FEMS Yeast Research. 2016, 16(4) : 1-11 Zhigang Chen, Jingkai Li, Wei Wang, Xiaoxian Guo, Yongquan Li, Xuming Mao, Xinyu Chen, Wenjun Guan*. Mutations Adjacent to the end of Transmembrane Helices 6 and 7 Independently Affect Drug Efflux Capacity of Yeast ABC Transporter Pdr5p. Biochimica et Biophysica Acta (BBA) - Biomembranes. 2014, 1838: 932–939. Hui Jiang, Yue-Yue Wang, Xin-Xin Ran, Wei-Ming Fan, Xin-Hang Jiang, Wen-Jun Guan*, and Yong-Quan Li*. Improvement of Natamycin Production by Engineering of Phosphopantetheinyl Transferases in Streptomyces chattanoogensis L10. Applied and Environmental Microbiology. 2013, 79: 3346-3354. Huifeng Jiang#, Wenjun Guan#, Zhenglong Gu*. Tinkering evolution of a post transcriptional RNA regulon: Puf3p in fungi as an example. PLoS Genetics. 2010, 6(7): e1001030. Huifeng Jiang, Wenjun Guan, David Pinney, Wen Wang and Zhenglong Gu*. Relaxation of yeast mitochondrial functions after whole-genome duplication, Genome Research. 2008,18:1466-1471. Wenjun Guan*, Yu Li, Yuquan Chen. Glucose biosensor based on multi-wall carbon nanotubes and screen-printed carbon electrodes. Biosensors & Bioelectronics. 2005, 21(3): 508-512.