入选国家、省等高级人才计划，先后负责国家蛋白质重大研究计划“青年 973 项目”、国家优秀青年基金项目，获得“树兰基金”医学青年人才奖、中美生物医学工程青年科学家奖(2019)(每 3 年 1 次， 每次中美各 1 人)等奖项;先后受邀在中国免疫学学会年会(2018)、全国生物力学大会(2018)和中美生物医学工程暨海内外生物力学研讨会(2019)上做大会主题报告。 长期致力于研究膜受体识别机理研究，突破生命科学领域传统研究手段的限制，自主研发了“多谱相”及“超稳”单分子力谱技术，在细胞膜原位精准表征物理-化学耦合调控下的膜蛋白识别机理及跨膜信号转导的规律，授权 9 项国家发明专利;先后主持了国家科技部蛋白质重大研究计划“青年项目(青年 973)”(2014)和国 家自然科学基金委“优秀青年项目” (2015)等项目，积极参与新冠疫情相关研究，利用单分子力谱技术，揭示了力学-化学耦合动态调控 T 细胞受体 (T-cell receptor, TCR)非我抗原识别以及新冠病毒入侵等分子机制，提出新型阻断新冠病毒入侵的策略，为阐明诸如重大疾病的免疫应答、新冠病毒感染等规 律及肿瘤免疫治疗、新冠疫情防治等提供重要的理论基础; 获得了一系列国内外同行认可的学术成绩。迄今在 Cell、Molecular Cell、Cell Research、JCB 等共发表学术论著 31 篇。近五年来，以通讯作者(含共同)在 Mol Cell (2019) (被 Faculty 1000 收录推荐)、Cell Res (三篇 2020; 2021;2024)、EMBO J (2021)、Angew Chemie (2023)、PNAS（2023）、JCB（2023）、eLife (2019)、Nano Lett (2020)、Bioinformatics (2021)、JITC (2022)等发表论文。 教育经历 1997-2001 浙江大学电气工程学院 本科 2001-2003 浙江大学电气工程学院 硕士 2003-2009 美国 Georgia Insitute of Technology PhD (Bioengineering)

膜蛋白动态识别及跨膜激活 单细胞及单分子生物医学工程 系统生物医学工程

研究领域 单分子及单细胞技术 | 力生物学与生物力学 | 膜受体动态结构调控 | 分子免疫学及免疫工程 | 分子神经生物学 | 系统生物学 研究方向 以生命医学重要问题为目标，注重传统理工科与生命科学、医学深度交叉, 在单分子、单细胞水平研究分子、细胞及系统动态调控机制。 感染与免疫学方向 病毒识别、免疫识别的力学动态机制及变革性药物研发； 生物工程方向 单分子蛋白质动态原位定量检测技术的研发及应用；单细胞检测技术；新型生物大分子及细胞药物研发； 力学生物学方向 力学动态调控蛋白质机器的结构和功能； 系统生物医学方向 重大疾病的单细胞免疫图谱及其在临床疾病诊疗中的应用； 神经生物学方向 神经突触突触形成和成熟的物理-化学耦联调控的分子机制；

I. Peer-reviewed papers (* co-first author, # co-correspoinding author): 2024 Targeting pro-inflammatory T cells as a novel therapeutic approach to potentially resolve atherosclerosis in humans, Lin Fan, Junwei Liu, Wei Hu, et al., Xinyang Hu#, Weiwei Yin#, Wei Chen#, Jian'an Wang#, Cell Research, 2024 (Cover Story) Volume 34 Issue 6 A Nian beast (proinflammatory PD-1+ T cells) wreaks havoc in the village (a metaphor for atherosclerosis, AS) on New Year's Eve. A man, dressed in red, ignites firecrackers (FcgR-binding anti-PD-1 mAb), preventing the Nian beast from causing trouble in the village (the shrinkage of AS plaques). 2023 Yajuan Yan, Ping Zhou, Lurong Ding, Wei Hu, Wei Chen#, Bin Su#，T Cell Antigen Recognition and Discrimination by Electrochemiluminescence Imaging, Angewandte Chemie，2023 Lina Zhou*, Shuai Wang*, Wei Hu*, Xiaoqian Liu, Lingdong Xu, Bolu Tong, Tongtong Zhang, Zhonghui Xue, Yixin Guo, Jing Zhao, Linrong Lu, Hengyu Fan, Wenbin Qian, Jian Chen, Wei Chen#, Lie Wang#，T cell proliferation requires ribosomal maturation in nucleolar condensates dependent on DCAF13, Journal of Cell Biology, 2023; Rui Qin*, Chenyi An*, Wei Chen，Physical-chemical regulation of membrane receptors dynamics in viral invasion and immune defense，Journal of Molecular Biology, 2023; Hui Chen*, Xinyi Xu*, Wei Hu*, Songfang Wu, Jianhui Xiao, Peng Wu, Xiaowen Wang, Xuling Han, Yanruo Zhang, Yong Zhang, Ning Jiang, Wanli Liu, Changjie Lou, Wei Chen#, Chenqi Xu#, Jizhong Lou#, Self-programmed dynamics of T cell receptor condensation, PNAS, 2023; 2022 Lin Fan*, Junwei Liu*, Yang Zhang*, Chenyun Zhang, Beisheng Shi, Xinyang Hu, Wei Chen#, Weiwei Yin#, Jian’an Wang#,High-dimensional Single-cell Analysis Delineates Peripheral Immune Signature of Coronary Atherosclerosis in Human Blood, Theranostics, 2022; Xuyong Wei*, Wentao Xie*, Weiwei Yin*, Mengfan Yang * , Abdul Rehman Khan , Renyi Su, Wenzhi Shu , Binhua Pan , Guanghan Fan , Kun Wang , Fan Yang , Di Lu , Changbiao Li , Linhui Pan , Beini Cen, Haiyang Xie , Li Zhuang , Shusen Zheng , Xun Zeng# , Wei Chen#, Xiao Xu#, Prediction of tumor recurrence by distinct immunoprofiles in liver transplant patients based on mass cytometry, American journal of cancer research, 2022; Yinxuan Lei*, Panyu Fei*, Bin Song, Wenjia Shi, Cheng Luo, Dahai Luo, Dan Li#, Wei Chen#, Jie Zheng#, A loosened gating mechanism of RIG-I leads to autoimmune disorders, Nucleic Acids Research, 2022; Jiawei Shi, Junwei Liu, Xiaoxuan Tu, Bin Li, Zhou Tong, Tian Wang, Yi Zheng, Hongyu Shi, Xun Zeng#, Wei Chen#, Weiwei Yin# and Weijia Fang#, Single-Cell immune signature for detecting early-stage HCC and early assessing anti-PD-1 immunotherapy efficacy, Journal of Immunotherapy for Cancer, 2022; (News Link); 2021 Juan Fan*, Jiawei Shi*, Yong Zhang*, Junwei Liu, Chenyi An, Huaying Zhu, Peng Wu, Wei Hu, Rui Qin, Danmei Yao, Xin Shou, Yibing Xu, Zhou Tong, Xue Wen, Jianpo Xu, Jin Zhang, Weijia Fang, Jizhong Lou#, Weiwei Yin#, Wei Chen#, NKG2D discriminates diverse ligands through selectively mechano‐regulated ligand conformational changes, EMBO J. , 2021; Panyu Fei, Haibo Ding, Yu Duan, Xinyi Wang, Wei Hu, Peng Wu, Mengxiao Wei, Zhengchun Peng#, Zhongze Gu#, Wei Chen#, Utility of TPP-manufactured biophysical restrictions to probe multiscale cellular dynamics, Bio-Design and Manufacturing, 2021; An Chenyi, Chen Wei, Multiplexed single-molecule force spectroscopy for dissecting biophysical regulation of membrane receptors functions on live cells, Biophysics Reports, 2021; Yan Shi#, Erdinc Sezgin#, Wei Chen#,The Role of Biomembranes and Biophysics in Immune Cell Signaling, Frontiers in Immunonlogy, 2021; Wei Hu*, Yong Zhang*, Panyu Fei*, Tongtong Zhang*, Danmei Yao*, Yufei Gao, Jia Liu, Hui Chen, Qiao Lu, Tenny Mudianto, Xinrui Zhang, Chuxuan Xiao, Yang Ye, Qiming Sun, Jing Zhang, Qi Xie, Pei-Hui Wang, Jun Wang#, Zhenhai Li#, Jizhong Lou#, Wei Chen#,Mechanical activation of spike fosters SARS-CoV-2 viral infection, Cell Research, 2021 (News Link); Left: Cell Research Cover: Kids (ACE2) pull the spike on the egg's surface (egg represents virion), monters inside the egg represents viral mRNA. Kids are lured to pull the spikes to help the viral invasion. Mid: Model of mechannical activation of SARS-CoV-2 spike and a novel neutralizing strategy. Right: Cell Research cover backup:Kid (ACE2) pulls the door knocker(SARS2-RBD) , helping to release a monters (SARS2-genome) by breaking up the threshold (S1/S2 lock); Junwei Liu, Lulu Liu, Saisi Qu, Tongtong Zhang, Danyang Wang, Qinghua Ji, Tian Wang, Hongyu Shi, Kaichen Song, Weijia Fang, Wei Chen#, Weiwei Yin#, GdClean: removal of Gadolinium contamination in mass cytometry data, Bioinformatics, 2021 （News Link); Tongtong Zhang*, Wei Hu*, Wei Chen, Plasma Membrane Integrates Biophysical and Biochemical Regulation to Trigger Immune Receptor Functions, Frontiers in Immunology 12, 53, 2021； J Liu, S Qu, T Zhang, Y Gao, H Shi, K Song, W Chen#, W Yin#, Applications of single-cell omics in tumor immunology, Frontiers in Immunology ,12, 2021;