学习经历 2000 年 09 月 - 2004 年 07 月，就读于山东大学物理学院物理学基地班，获学士学位。 2004 年 09 月 - 2009 年 07 月，在中国科学院物理研究所攻读软物质物理与生物物理专业，获博士学位。 工作经历与任职情况 2009 年 07 月 - 2010 年 03 月，任职于国家知识产权局，担任专利审查员。 2010 年 03 月 - 2015 年 10 月，在新加坡 - MIT 联合科研中心生物系统与微观力学实验室担任研究科学家。 2015 年 11 月 - 2020 年 8 月，担任武汉大学生命科学学院、武汉大学高等研究院研究员、博士生导师。 2020 年 9 月 - 至今，担任武汉大学生命科学学院教授、博士生导师。

使用单分子磁镊技术和单分子FRET技术，揭示RNA、DNA、蛋白质等生物大分子的动态结构及其相互作用。通过结构生物信息学方法，探索生物大分子的结构动态和功能机制。我们还发展了DNA力学探针，用于临床场景的分子检测和单分子细胞力学检测。当前的研究重点包括DNA甲基化、单分子检测技术、核小体和染色质的动态调控。

通讯作者论文： 1. Yuru Hu#, Hongyun Li#*, Chen Zhang#, Jingjing Feng#, Wenxu Wang, Wei Chen, Miao Yu, Xinghua Zhang*, Zheng Liu*, "DNA-based ForceChrono Probes for Deciphering Single-Molecule Force Dynamics in Living Cells", Cell, 187(13), 1–15 (2024) 2. Chen Zhang#, Fu-Jia Tian#, Hong-Wei Zuo#, Wei Wei, Qi-Yuan Qiu, Zhi-Jie Tan, Yan Zhang*, Wen-Qiang Wu, Liang Dai* and Xing-Hua Zhang*, "Counterintuitive DNA destabilization by monovalent salt at high concentrations", Nature Communications in press (2024) 3. Yun-Long Chen* and Xing-Hua Zhang*, "Effects of imidazolium-based ionic liquids on the elasticity of DNA revealed by magnetic tweezers", Journal of Molecular Liquids, 410: 125646 (2024) 4. Er-Chi Zhou#, Hang Fu#, Hao-Ze Wang, Ya-Jun Yang, Xing-Hua Zhang*, "Converting multiple to single DNA-tethered beads and removing one-end-tethered only DNA in high-throughput stretching", ACS Sensors, https://doi.org/10.1021/acssensors.4c02585 (2024) 5. Bohua Yin, Shaoxi Fang, Bin Wu, Wenhao Ma, Daming Zhou, Yajie Yin, Rong Tian, Shixuan He, Wanyi Xie*, Xinghua Zhang*, Zuobin Wang*, Deqiang Wang*, "Directly Characterizing the Capture Radius of Tethered Double-Stranded DNA by Single-Molecule Nanopipette Manipulation", ACS Nano 18(40): 27962−27973 (2024) 6. Chen-Chen Zheng#, Yun-Long Chen#, Hai-Long Dong, Xing-Hua Zhang*, Zhi-Jie Tan*, "Effect of ethanol on the elasticities of double-stranded RNA and DNA revealed by magnetic tweezers and simulations", The Journal of Chemical Physics 161: 075101 (2024) 7. Peng Chen#, Jin Zhou#, Huan Liu#, Erchi Zhou#, Boxiao He, Yankang Wu, Hongjian Wang, Zaiqiao Sun, Chonil Paek, Jun Lei, Yongshun Chen*, Xinghua Zhang*, Lei Yin*, “Engineering of Cas12a nuclease variants with enhanced genome-editing specificity”, PLoS Biology, 22(3): e3002514 (2024) 8. Hai-Long Dong#, Chen Zhang#, Liang Dai, Yan Zhang*, Xing-Hua Zhang*, and Zhi-Jie Tan*, “The origin of different bending stiffness between double-stranded RNA and DNA revealed by magnetic tweezers and simulations.”, Nucleic Acids Research, 52(5):2519-2529 (2024) 9. Ya-Jun Yang, Hang Fu, Xiao-Lu Li, Hong-Yu Yang, Er-Chi Zhou, Cheng-Yu Xie, Shu-Wen Wu, Fan He*, Yan Zhang* and Xing-Hua Zhang*, “A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.”, Nucleic Acids Research, 51(17): e90 (2023) 10. Fu-Jia Tian#, Chen Zhang #, Erchi Zhou#, Hai-Long Dong, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*， “Universality in RNA and DNA deformations induced by salt, temperature change, stretching force, and protein binding.” PNAS, 120 (20) e2218425120 (2023) 11. Xiao-Cong Zhao#, Hai-Long Dong#, Xiao-Lu Li, Hong-Yu Yang, Xue-Feng Chen, Liang Dai, Wen-Qiang Wu, Zhi-Jie Tan* and Xing-Hua Zhang*, “5-methyl-cytosine stabilizes DNA but hinders DNA hybridization revealed by magnetic tweezers and simulations.” Nucleic Acids Research, 50 (21): 12344–12354 (2022) 12. Chen Zhang#, Fujia Tian#, Ying Lu, Bing Yuan, Zhi-Jie Tan, Xing-Hua Zhang* and Liang Dai*, “Twist-diameter coupling drives DNA twist changes with salt and temperature.” Science Advances, 8(12), abn1384 (2022) 13. Xiao-Wei Qiang#, Chen Zhang#, Hai-Long Dong#, Fu-Jia Tian, Hang Fu, Ya-Jun Yang, Liang Dai, Xing-Hua Zhang*, Zhi-Jie Tan*, “Multivalent cations reverse the twist-stretch coupling of RNA.” Physical Review Letters, 126, 108103. (2022) 14. Xuejie Wang#, Yang Dong#, Xiaocong Zhao#, Jinbao Li, Jordan Lee, Zhenxin Yan, Shuangshuang Yang, Wenqiang Wu, Ximiao Hou, Guangxue Liu, Yueyue Zhang, Lun Song, Gang Cai, Qing Li, Grzegorz Ira, Xinghua Zhang*, Xuefeng Chen*, “Rtt105 promotes high-fidelity DNA replication and repair by regulating the single-stranded DNA binding factor RPA.” PNAS, 118 (25): e2106393118 (2021) 15. Ya-Jun Yang, Hai-Long Dong, Xiao-Wei Qiang, Hang Fu, Er-Chi Zhou, Chen Zhang, Lei Yin, Xue-Feng Chen, Fu-Chao Jia, Liang Dai, Zhi-Jie Tan, and Xing-Hua Zhang*. “Cytosine methylation enhances DNA condensation revealed by equilibrium measurements using magnetic tweezers”. Journal of the American Chemical Society. 142 (20): 9203–9209 (2020) 16. Hang Fu#, Chen Zhang#, Xiao-Wei Qiang#, Ya-Jun Yang, Zhi-Jie Tan* and Xing-Hua Zhang*. “Opposite effects of high-valent cations on the elasticities of DNA and RNA duplexes revealed by magnetic tweezers.” Physical Review Letters. 124(5): 058101 (2020) 17. Chen Zhang#, Hang Fu#, Yajun Yang#, Erchi Zhou, Zhijie Tan, Huijuan You, Xinghua Zhang*. “The Mechanical Properties of RNA-DNA Hybrid Duplex Stretched by Magnetic Tweezers”. Biophysical Journal. 116 (2): 196-204 (2019) (Selected as the best paper of 2019)