2011-至今 武汉大学药学院 教授 2008-2011 美国麻省理工学院生物工程系 博士后 2006-2008 美国麻省理工学院生物工程系 访问博士生 2003-2008 上海交通大学生命科技学院 硕博连读 1999-2003 山东大学生命科学院 本科

1. 基因组表观遗传修饰与合成生物学（Genomic phosphorothioate modification and synthetic biology） 作为一种新型的DNA表观遗传现象——DNA磷硫酰化修饰于2007年被鉴定，被《环球科学》评为当年“十大科学新闻”。不同于常见的甲基化、羟甲基化等碱基修饰，磷硫酰化修饰发生在DNA骨架上，即细菌和古菌能够利用DndABCDE 5 个蛋白对DNA磷酸二酯键中的非桥连氧原子进行硫取代，形成序列特异性、空间构象专一性的磷硫酰化结构。不仅如此，本实验室还发现了全新的II型Ssp磷硫酰化限制修饰系统。磷硫酰化生理修饰具有动态修饰特征，在原核生物和环境、人体微生物组中广泛分布，并进化出多样的生理功能，包括参与限制-修饰防御、基因的表达调控、参与维持细胞内氧化-还原动态平衡等，可以与细胞中其它的防御系统组成防御网络，并可构建抗噬菌体的合成生物学工程菌底盘。 2. 表观遗传修饰的生物医学应用（Epigenetic and biomedical applications） 表观修饰拓展了DNA的遗传信息，在细胞生命活动中起到了不可或缺的关键作用。我们致力于研究包含基因组磷硫酰化和甲基化在内的多种表观修饰对病原菌和病毒的致病性、细胞的生理及代谢的影响、以及相关的生物医学领域应用。由于抗生素的滥用，超级耐药菌的出现是现代医学面临的一个重大挑战。研究表观遗传修饰对耐药菌耐药机制所产生的影响，以及发展相应的小分子药物，并结合噬菌体疗法，解析噬菌体防治超级耐药菌的机制，为超级耐药病原菌的防治提供理论和应用基础。

1. Xiaolin Xiong, Geng Wu, Yue Wei, Liqiong Liu, Yubing Zhang, Rui Su, Xianyue Jiang, Mengxue Li, Haiyan Gao, Xihao Tian, Yizhou Zhang, Li Hu, Si Chen, You Tang, Susu Jiang, Ruolin Huang, Zhiqiang Li, Yunfu Wang, Zixin Deng, Jiawei Wang, Peter C. Dedon, Shi Chen, Lianrong Wang*. SspABCD-SspE is a phosphorothioation-sensing bacterial defense system with broad antiphage activities, Nature Microbiology, 2020, July, 5: 917-928. DOI: 10.1038/s41564-020-0700-6. 2. Dan Wu#, Yaqian Tang, Siwei Chen, Yue He, Xiaofei Chang, Wenzhong Zheng, Zixin Deng, Zhiqiang Li, Lianrong Wang*, Geng Wu* & Shi Chen*. The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex. Nature Catalysis. 2022, December, Volume 5, 1131–1144. 3. Xiaolin Wu#, Bo Cao#, Patricia Aquino, Tsu-Pei Chiu, Chao Chen, Susu Jiang, Zixin Deng, Shi Chen, Remo Rohs, Lianrong Wang*, James E Galagan*, Peter C Dedon*. Epigenetic Competition Reveals Density-Dependent Regulation and Target Site Plasticity of Phosphorothioate Epigenetics in Bacteria. Proc Natl Acad Sci U S A. 2020, June, 117 (25):14322-14330. DOI: 10.1073/pnas.2002933117. 4. Xuan Zou#, Xiaohong Xiao, Ziran Mo, Yashi Ge, Xing Jiang, Ruolin Huang, Mengxue Li, Zixin Deng, Shi Chen*, Lianrong Wang*, Sang Yup Lee*. Systematic strategies for developing phage resistant Escherichia coli strains. Nature Communications, 2022, 13: 4491. 5. Haiyan Gao, Xinqi Gong, Jinchuan Zhou , Yubing Zhang, Jinsong Duan, Yue Wei, Liuqing Chen, Zixin Deng, Jiawei Wang, Shi Chen*, Geng Wu *, Lianrong Wang*. Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE. Nature Communications, 2022, November, 13:6773. 6. Ming-Yu Wu#, Luojia Chen, Qingrong Chen, Rui Hu, Xiaoyu Xu, Yifei Wang, Jie Li, Shun Feng, Changjiang Dong, Xiao-Lian Zhang, Zhiqiang Li, Lianrong Wang,* Shi Chen,* and Meijia Gu*. Engineered Phage with Aggregation-Induced Emission Photosensitizer in Cocktail Therapy against Sepsis. Advanced Materials. 2023, 35, 2208578, DOI: 10.1002/adma.202208578. 7. Ming-Yu Wu#, Xiaoyu Xu#, Rui Hu#, Qingrong Chen, Luojia Chen, Yuncong Yuan, Jie Li, Li Zhou, Shun Feng, Lianrong Wang*, Shi Chen*, Meijia Gu*. A Membrane-Targeted Photosensitizer Prevents Drug Resistance and Induces Immune Response in Treating Candidiasis. Advanced Science. 2023; e2207736. doi: 10.1002/advs.202207736. 8. Yue Wei#, Qinqin Huang, Xihao Tian, Mingmin Zhang, Junkai He, Xingxiang Chen, Chao Chen, Zixin Deng, Zhiqiang Li, Shi Chen, and Lianrong Wang*, Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics. Nucleic Acids Research, 2021, Doi: 10.1093/nar/gkab169. 9. Ming-Yu Wu, Meijia Gu, Jong-Kai Leung, Xinmei Li, Yuncong Yuan, Chao Shen, Lianrong Wang*, Engui Zhao*, Sijie Chen*. A Membrane-Targeting Photosensitizer with Aggregation-Induced Emission Characteristics for Highly Efficient Photodynamic Combat of Human Coronaviruses. Small, 2021, June, https://doi.org/10.1002/smll.202101770. 10. Bo Cao#, Chao Chen#, Michael S. DeMott#, Qiuxiang Cheng, Tyson A. Clark, Xiaolin Xiong, Xiaoqing Zheng, Vincent Butty, Stuart S. Levine, George Yuan, Matthew Boitano, Khai Luong, Yi Song, Xiufen Zhou, Zixin Deng, Stephen W. Turner, Jonas Korlach, Delin You*, Lianrong Wang*, Shi Chen*, Peter C. Dedon. Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences. Nature Communications. 2014, 5: 3951. 11. Wenzhong Zheng#, Kexin Wang#, Yachen Wu#, Ge Yan, Chi Zhang, Zhiqiang Li, Lianrong Wang*, Shi Chen*. C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2α. Theranostics. : 2022, Oct, 17, , Vol. 12, Issue 17. 12. You Tang#, Yue Hong, Li Liu, Xuan Du, Yaqi Ren, Susu Jiang, Wanjing Liu, Chen Chao, Zixin Deng, Lianrong Wang*, Shi Chen*. Involvement of the DNA Phosphorothioation System in TorR Binding and Anaerobic TMAO Respiration in Salmonella enterica. mBio, 2022, April 14, Vol. 13, No. 3. 13. He W#, Gao H#, Wu D, Jiang S, Huang W, Chen C, Deng Z, Xiong L, Wu G*, Wang L*. Structural and Functional Analysis of DndE Involved in DNA Phosphorothioation in the Haloalkaliphilic Archaea Natronorubrum bangense JCM10635. mBio. 2022 Apr 14:e0071622. doi: 10.1128/mbio.00716-22. 14. Pengqing Nie#, Zhiqiang Li#, Yimeng Wang, Yubing Zhang, Mengna Zhao, Jie Luo, Shiming Du, Zixin Deng, Jincao Chen, Yunfu Wang, Shi Chen, Lianrong Wang*. Gut microbiome interventions in human health and diseases. Medicinal Research Reviews, 2019 Apr 17. doi: 10.1002/med.21584. 15. Yajie Wang# , Zhikang Miao# , Chang Xu, Ying Cai, Yuting Yang, Yue Hu, Mengna Zhao, Yue Shao, Zhiqiang Li, Jincao Chen, Shi Chen*, Lianrong Wang*. Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats. Cell Death & Disease, 2023 May 13;14(5):325. doi: 10.1038/s41419-023-05846-5. 16. Meijia Gu#,*, Luojia Chen#, Rui Hu, Qingrong Chen, Jianbo Liu*, Lianrong Wang*, Shi Chen*. Aggregation-induced emission: recent applications in infectious diseases. SCIENCE CHINA Chemistry, 2023 Oct 24. doi.org/10.1007/s11426-023-1825-7. 17. Lianrong Wang#, Shi Chen#, Tiegang Xu, Koli Taghizadeh, John S Wishnok, Xiufen Zhou, Delin You, Zixin Deng*, Peter C Dedon*. Phosphorothioation of DNA in bacteria by dnd genes. Nature Chemical Biology. 2007;3(11):709-10. 18.Lianrong Wang, Shi Chen*, Kevin L. Vergin, Stephen J. Giovannoni, Simon W. Chan, Michael S. DeMott, Koli Taghizadehe, Otto X. Cordero, Michael Cutler, Sonia Timberlake, Eric J. Alm, Martin F. Polz, Jarone Pinhassi, Zixin Deng, Peter C. Dedon*. DNA phosphorothioation is widespread and quantized in bacterial genomes. Proc Natl Acad Sci USA. 2011; 108(7):2963-8.