孔蕴 照片

孔蕴

副教授

所属大学: 山东大学

所属学院: 国家糖工程技术研究中心

邮箱:
kongyun@sdu.edu.cn

个人主页:
http://www.glycoeng.sdu.edu.cn/info/1059/1080.htm

个人简介

教育经历 2011/02-2014/10,哥本哈根大学,健康学院,细胞与遗传医学博士 2007/09-2010/07,山东大学,生命科学学院,微生物学硕士 2003/09-2007/07,山东大学,国家生命科学与技术人才培养基地,学士 研究经历 2020/09-今,山东大学,国家糖工程技术研究中心,副教授 2015/01-2020/9,山东大学,国家糖工程技术研究中心,助理研究员

研究领域

结合基因编辑技术和高分辨率质谱的“蛋白质糖基化修饰”的分析与功能学研究; 多糖与糖蛋白疫苗设计与生产研究; 单克隆抗体等生物大分子药物的糖基化工程研究。

近期论文

【1】 Yongheng Rong# , Xingyun Wang, Weian Mao, Min Chen, Shengjun Wang , Peng George Wang*, Yunjiao He*, Yun Kong(孔蕴)*. O-GalNAc glycosylation affects the immunogenicity of the receptor-binding domain (RBD) of SARS-CoV-2 spike protein, Chem Commun (Camb), 2023 Feb 9;59(13):1797-1800. 【2】 Yongheng Rong#, Xingyun Wang, Weian Mao, Fang Yuan, Min Chen, Shengjun Wang, Peng George Wang, Zhigang Wu, Yunjiao He*, and Yun Kong(孔蕴)*. Chemoenzymatic Synthesis of SARS-CoV‑2 Homogeneous O‑Linked Glycopeptides for Exploring Their Inhibition Functions, ACS Infect. Dis, 2022, 8, 2198−2206. 【3】 Yun Kong(孔蕴)#, Fang Liu#, Zhaoxi Liu, Jiayu Zhao, Qizheng Wu, Xunlian Zhang, Min Liu, Houcheng Zhang, Songqiang Liu, Xiaomei Zhang*, Min Chen*. Synthesis of globotriose-modified peptides for the preparation of a colorimetric biosensor to detect Shiga toxins, Talanta, 2022, 243. 【4】 Li Kun#, Liu Zhaoxi, Liu Xiaoyu, Wang Lei, Zhao Jiayu, Zhang Xunlian, Kong Yun(孔蕴)*, Chen Min*. An anti-biofilm material: polysaccharides prevent the precipitation reaction of silver ions and chloride ions and lead to the synthesis of nano silver chloride, Nanotechnology, 2021, 32(31). 【5】 Shengjun Wang#, Yongheng Rong, Yaoguang Wang, Decai Kong, Peng George Wang, Min Chen, Yun Kong(孔蕴)*. Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris, Microbial Cell Factories, 2020, 19(7). 【6】 Xunlian Zhang#, Weihao Sheng, Kun Li, Yongheng Rong, Qizheng Wu, Qingyun Meng, Yun Kong(孔蕴)*, Min Chen*. Substrate specificity of the galactokinase from the human gut symbiont Akkermansia muciniphila ATCC BAA-835, Enzyme and Microbial Technology, 139 (2020) 109568. 【7】 Qingzheng Wu#, Fang Liu, Yingjuan Song, Qingyun Meng, Xunlian Zhang, Huizi Li, Jingyu Sun, Hongqi Gao, Peng George Wang, Yun Kong(孔蕴)*, Min Chen*. A new strategy for high-throughput identification of human gut microbiota containing specific monosaccharide, Sci China Life Sci, 62(2019) 985-987. 【8】 Qingyun Meng#, Kun Li, Yongheng Rong, Qizheng Wu, Xunlian Zhang, Yun Kong(孔蕴)*, Min Chen*. Probing peptide substrate specificities of N-glycosyltranferase isoforms from different bacterial species, Carbohydrate Research, 473 (2019) 82–87. 【9】 Shengjun Wang#, Yaoguang Wang#, Peng George Wang, Min Chen, Yun Kong(孔蕴)*. High level expression and glycosylation of recombinant Mycobacterium tuberculosis Ala-Pro-rich antigen in Pichia pastoris, Protein Expression and Purification, 150 (2018) 67–71. 【10】 Yun Kong(孔蕴)#, Jiang Li, Xinyuan Hu, Yaoguang Wang, Qingyun Meng, Guofeng Gu, Peng George Wang, Min Chen*. N-Glycosyltransferase from Aggregatibacter aphrophilus synthesizes glycopeptides with relaxed nucleotide-activated sugar donor selectivity, Carbohydrate Research. 462 (2018) 7-12. 【11】 Yun Kong(孔蕴)#,Yajun Qu, Shengjun Wang , Peng George Wang, Min Chen*. Heterologous expression of Shigella dysenteriae serotype 1 O-antigen analog in Escherichia coli K-12 W3110 by transferring a minimum number of genes involved in O-polysaccharide biosynthesis, Biotechnology Letters, 40(2018) 1219–1226. 【12】 Yun Kong(孔蕴)#, Hiren J Joshi, Katrine Ter-Borch Gram Schjoldager, Thomas Daugbjerg Madsen, Thomas A Gerken, Malene B Vester-Christensen, Hans H Wandall, Eric Paul Bennett, Steven B Levery, Sergey Y Vakhrushev, Henrik Clausen*. Probing polypeptide GalNAc-transferase isoform substrate specificities by in vitro analysis, Glycobiology, 2015, 25(1): 55-65.