顾景凯
教授
个人简介
教育经历 1997年至2000年 吉林大学超分子结构与材料国家重点实验室,博士后。 1994年至1997年 沈阳药科大学药学院,药物分析,博士; 工作经历 现任吉林大学唐敖庆特聘教授;兼任吉林大学艾滋病疫苗国家工程实验室、分子酶学工程教育部重点实验室,教授。 2012-01-至今,吉林大学,唐敖庆特聘教授 2003-12-至今,吉林大学,生命科学学院药物代谢研究中心主任,教授,博士生导师 2001-09,吉林大学,破格晋升为“激励岗”教授 2000-01至2001-09,吉林大学,生命科学学院,副教授 学术兼职 兼任国家药品监督管理局药品注册审评专家咨询委员会专家;中国医药质量管理协会临床研究质量与评价专业委员会,副主任委员;国家药品监督管理局“药用辅料质量研究与评价重点实验室”学术委员会,委员;国家中医药管理局“中药药代动力学三级实验室”,主任;中国生化制药协会专家委员会,委员;全国工商联医药业商会专家;中国药理学会:药物代谢专业委员会、临床药理专业委员会、药物基因组专业委员会、分析药理专业委员会,常务委员;中国药学会:药物分析专业委员会、应用药理学会专业委员会,常务委员;中国医药生物技术协会药物分析技术分会,常务委员;吉林省药理学会副理事长;中国质谱学会有机质谱专业委员会,委员;美国化学会(ACS),会员;国际药物及化学异物代谢学会(ISSX),会员。
研究领域
药物代谢动力学;体内药物分析;生物质谱分析;靶点药物分析;纳米药物体内多形态成分分析;多糖、ADC及蛋白肽类等生物大分子药物分析;高分子药用辅料质量控制与体内评价;新型给药系统的体内时空命运研究;基于DMPK的新药设计与开发
近期论文
1.Bai, Ruifeng,Sun ,Dong,Shan, Yuqin, Guo, Zhiqiong, Chu, Dafeng , Fawcett, John Paul, Gu, Jingkai*,Disposition and fate of polyoxyethylene glycerol ricinoleate as determined by LC-Q-TOF MS coupled with MSALL, SWATH and HR MS/MS techniques. CHINESE CHEMICAL LETTERS (32) 3237-3240,2021,10 2.Meng, Xiangjun, Zhang, Zhi,Tong, Jin, Sun, Hui, Fawcett, John Paul, Gu, Jingkai* The biological fate of the polymer nanocarrier material monomethoxy poly(ethylene glycol)-block-poly(D,L-lactic acid) in rat. ACTA PHARMACEUTICA SINICA B (11):1003-1009,2021 .4 3. Zhang Z, Jiang H, Zhang Y, Zhang D, Fawcett JP, Gu J*. Comprehensive Bioanalysis of Ultrahigh Molecular Weight, Highly Disperse Poly(ethylene oxide) in Rat via Microsolid Phase Extraction and RPLC-Q-Q-TOF Coupled with the MSALL Technique. Anal Chem. 2020; doi: 10.1021/acs.analchem.0c00189. 4. Zhang Z, Zhang Y, Song S, Yin L, Sun D, Gu J*. Recent advances in the bioanalytical methods of polyethylene glycols and PEGylated pharmaceuticals. J Sep Sci. 2020; doi: 10.1002/jssc.201901340. 5. Wang T, Guo Y, He Y, Ren T, Yin L, Fawcett JP, Gu J*, Sun H*. Impact of molecular weight on the mechanism of cellular uptake of polyethylene glycols (PEGs) with particular reference to P-glycoprotein. Acta Pharm Sin B. 2020; doi:10.1016/j.apsb.2020.02.001 6. Wang H, Zheng M, Gao J, Wang J, Zhang Q, Fawcett JP, He Y*, Gu J*. Uptake and release profiles of PEGylated liposomal doxorubicin nanoparticles: A comprehensive picture based on separate determination of encapsulated and total drug concentrations in tissues of tumor-bearing mice. Talanta.2020; 208:120358. 7. Yin L, Ren T, Zhao S, Shi M*, Gu J*. Comparative pharmacokinetic study of PEGylated gemcitabine and gemcitabine in rats by LC-MS/MS coupled with pre-column derivatization and MSALL technique. Talanta. 2020; 206: 120184. 8. Su C, Liu Y, Li R, Wu W, Fawcett JP, Gu J*. Absorption, distribution, metabolism and excretion of the biomaterials used in Nanocarrier drug delivery systems. Adv Drug Deliv Rev. 2019; 143: 97-114. 9. Yin L, Zhang Z, Liu Y, Gao Y, Gu J*. Recent advances in single-cell analysis by MSspectrometry. Analyst. 2019;144:824-845. 10. Yin L, Feng Y, Tong J, Guo Z, Zhang Y, Zhang Q, Sun Y, Fawcett JP, Gu J*. Ultrahigh-throughput absolute quantitative analysis of linezolid in human plasma by direct analysis in real time mass spectrometry without chromatographic separation and its application to a pharmacokinetic study. Anal Bioanal Chem. 2019;411:5139-5148. 11. Wang T, Yin L, Wang H, Fawcett JP, Gu J*. Development and application of a high-throughput liquid chromatography-tandem mass spectrometric method for the simultaneous determination of thymosin α1 and its recombinant human form in plasma and urine. J Pharm Biomed Anal. 2019;170:16-21. 12. Ren T, Zhang Z, Fawcett JP, Sun D*, Gu J*. Micro-solid phase extraction and LC-MS3 for the determination of triptorelin in rat plasma and application to a pharmacokinetic study. J Pharm Biomed Anal. 2019;166:13-19. 13. Zhang Z, Li R, Liu S, Yin L, Xu T, Fawcett JP, Gu J*. Liquid chromatography tandem mass spectrometry with triple stage fragmentation for highly selective analysis and pharmacokinetics of alarelin in rat plasma. J Sep Sci. 2019;42:3033-3040. 14. Liang C, Sun H, Meng X, Yin L, Fawcett JP, Yu H, Liu T, Gu J*. Separation and simultaneous quantitation of PGF2α and its epimer 8-iso-PGF2α using modifier-assisted differential mobility spectrometry tandem mass spectrometry. Acta Pharm Sin B. 2018;8:228-234. 15. Lin W, Yin L, Sun T, Wang T, Xie Z, Gu J*, Jing X*. The Effect of Molecular Structure on Cytotoxicity and Antitumor Activity of PEGylated Nanomedicines. Biomacromolecules. 2018;19:1625-1634. 16. Su C, Liu Y, He Y*, Gu J*. Analytical methods for investigating in vivo fate of nanoliposomes: A review. J Pharm Anal. 2018;8:219-225. 17. Sun D, Su C, Liu Y, Meng X, Fawcett JP, Guo Y, Gu J*. A Unique Collision-Induced Dissociation Reaction of Cholamine Derivatives of Certain Prostaglandins. J Am Soc Mass Spectrom. 2018;29:2360-2367. 18. Sun D, Meng X, Ren T, Fawcett JP, Wang H, Gu J*. Establishment of a Charge Reversal Derivatization Strategy to Improve the Ionization Efficiency of Limaprost and Investigation of the Fragmentation Patterns of Limaprost Derivatives Via Exclusive Neutral Loss and Survival Yield Method. J Am Soc Mass Spectrom. 2018; 29:1365 -1375. 19. Su C, Yang H, Sun H, Fawcett JP, Sun D*, Gu J*. Bioanalysis of free and liposomal Amphotericin B in rat plasma using solid phase extraction and protein precipitation followed by LC-MS/MS. J Pharm Biomed Anal. 2018;158:288-293. 20. Ren T, Li R, Meng X, Fawcett JP, Sun D*, Gu J*. Differential mobility spectrometry followed by tandem mass spectrometry with multiple ion monitoring for bioanalysis of eptifibatide in rat plasma. J Pharm Biomed Anal. 2018 ;151:260-265. 21. Zhou X, Meng X, Cheng L, Su C, Sun Y, Sun L, Tang Z, Fawcett JP, Yang Y*, Gu J*. Development and Application of an MSALL-Based Approach for the Quantitative Analysis of Linear Polyethylene Glycols in Rat Plasma by Liquid Chromatography Triple-Quadrupole/Time-of-Flight Mass Spectrometry. Anal Chem. 2017;89:5193-5200. 22. Cong Y, Hu L*, Zhang Z, Gao Y, Dong M, Qin H, Ye M*, Gu J, Zou H. Analysis of therapeutic monoclonal antibody glycoforms by mass spectrometry for pharmacokinetics study.Talanta,2017, 165:664-670. 23. Yin L, Su C, Ren T, Meng X, Shi M, Paul Fawcett J, Zhang M, Hu W, Gu J*. MSALL strategy for comprehensive quantitative analysis of PEGylated-doxorubicin, PEG and doxorubicin by LC-high resolution q-q-TOF mass spectrometry coupled with all window acquisition of all fragment ion spectra. Analyst. 2017 ;142:4279-4288. 24. Su C, Sun H, Yang H, Yin L, Zhang J, Fawcett JP, Gu J*. A validated UPLC-MS/MS method coupled with protein precipitation and ion exchange solid phase extraction for the quantitation of porcine relaxin B29 in dog plasma and its application to a pharmacokinetic study. Anal Bioanal Chem. 2017;409:6559-6565. 25. Meng X, Xu H, Zhang Z, Fawcett JP, Li J, Yang Y*, Gu J*. Differential mobility spectrometry tandem mass spectrometry with multiple ion monitoring for the bioanalysis of liraglutide. Anal Bioanal Chem. 2017;409:4885-4891. 26. Sun H, Zhang Q, Zhang Z, Tong J, Chu D*, Gu J*. Simultaneous quantitative analysis of polyethylene glycol (PEG), PEGylated paclitaxel and paclitaxel in rats by MS/MSALL technique with hybrid quadrupole time-of-flight mass spectrometry. J Pharm Biomed Anal. 2017;145:255-261. 27. Su C, Yang H, Meng X, Fawcett JP, Cao J, Yang Y*, Gu J*. Determination of rabeprazole enantiomers in dog plasma by supercritical fluid chromatography tandem mass spectrometry and its application to a pharmacokinetic study. J Sep Sci, 2017, 40:1010- 1016. 28. Lu H, Su C, Yin L, Gu L, Gu J*, Chen X*. Liquid chromatography-tandem mass spectrometry method for simultaneous determination of valproic acid and its ene-metabolites in epilepsy patient plasma. J Pharm Anal. 2016; 6: 112-116. 29. Yang Z, Xu X, Sun L, Zhao X, Wang H, Fawcett JP, Yang Y*, Gu J*. Development and validation of an enantioselective SFC-MS/MS method for simultaneous separation and quantification of oxcarbazepine and its chiral metabolites in beagle dog plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2016, 1020: 36-42. 29. Sun H, Wang J, Sun Y, Peng W, Sun L, Yang Y*, Gu J*. Rapid and sensitive liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of yonkenafil and its major metabolites in rat plasma. J Sep Sci. 2016, 39:3700-3708. 30. Yang Z, Sun L, Liang C, Xu Y, Cao J, Yang Y*, Gu J. Simultaneous quantitation of the diastereoisomers of scholarisine and 19-epischolarisine, vallesamine, and picrinine in rat plasma by supercritical fluid chromatography with tandem mass spectrometry and its application to a pharmacokinetic study. J Sep Sci. 2016, 39:2652-60. 31. Yang Z, Wang L, Xu M, Gu J, Yu L, Zeng S*. Simultaneous analysis of gemfibrozil, morphine, and its two active metabolites in different mouse brain structures using solid-phase extraction with ultra-high performance liquid chromatography and tandem mass spectrometry with a deuterated internal standard. J Sep Sci. 2016; 39:2087-96. 32. Yin L, Wang T, Shi M, Zhang Y, Zhao X, Yang Y*, Gu J*. Simultaneous determination of ten antiepileptic drugs in human plasma by liquid chromatography and tandem mass spectrometry with positive/negative ion-switching electrospray ionization and its application in therapeutic drug monitoring. J Sep Sci. 2016,39:964-72.