雷春阳
副教授 硕导
个人简介
教育背景 2008.9-2014.9 湖南大学 分析化学 理学博士 2004.9-2008.6 湖南大学 生物技术 理学学士 工作履历 2020.01-至今 湖南大学化学化工学院 副教授,硕士生导师 2017.03-2019.12 湖南大学化学化工学院 助理教授,硕士生导师 2014.10-2017.03 浙江大学生工食品学院 助理研究员/博士后
研究领域
从事基于酶分子工具创建的生物分析新方法研究
近期论文
K. Wang, S. Liu, S. Zhou, A. Qileng, D. Wang, Y. Liu, C. Chen, C. Lei*, Z. Nie*. Ligand-responsive artificial protein-protein communication for field-deployable cell-free biosensing. Angew. Chem. Int. Ed., 2024, doi: 10.1002/anie.202416671. B. Xu#, H. Tian#, X. Li, Q. Hao, Y. Ma, L. Liu, C. Lei*, Y. Chen*, Zhou Nie*. On-chip engineered living materials as field-deployable biosensing laboratories for multiplexed detection. Adv. Funct. Mater., 2024, doi: 10.1002/adfm.202416830. W. Kang, F. Xiao, X. Zhu, X. Ling, S. Xie, R. Li, P. Yu, L. Cao, C. Lei*, Y. Qiu, T. Liu, Z. Nie*. Engineering anti-CRISPR proteins to create CRISPR-Cas protein switches for activatable genome editing and viral protease detection. Angew. Chem. Int. Ed., 2024, 63, e20240599. S. Xie, C. Zhu, L. Yang, H. Li, H. Zhu, Z. Nie, C. Lei*. Programmable proteolysis-activated transcription for highly sensitive ratiometric electrochemical detection of viral protease. Anal. Chem., 2023, 95, 10728–10735. S. Chen, B. Gong, C. Zhu, S. Xie, C. Lei*, Z. Nie*. Nucleic acid-assisted CRISPR-Cas systems for advanced biosensing and bioimaging. TrAC-Trends Anal. Chem., 2023, 159, 116931. S. Wang#, Z. Zhang#, R. Tang, F. He, Y. Huang, Z. Nie, C. Lei*. Responsive MXene nanovehicles deliver CRISPR/Cas12a for boolean logic-controlled gene editing. Sci. China Chem., 2022, 65, 2318–2326. S. Xie, B. Xu, R. Tang, S. Chen, C. Lei*, Z. Nie*. Kinetics accelerated CRISPR-Cas12a enabling live-cell monitoring of Mn2+ homeostasis. Anal. Chem., 2022, 94, 10159–10167. W. Kang, L. Liu, P. Yu, T. Zhang, C. Lei*, Z. Nie*. A switchable Cas12a enabling CRISPR-based direct histone deacetylase activity detection. Biosens. Bioelectron., 2022, 213, 114468. S. Chen, R. Wang, S. Peng, S. Xie, C. Lei*, Y. Huang, Z. Nie*. PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing. Chem. Sci., 2022, 13, 2011–2020.(ESI高被引论文) F. Liu, R. Chen, W. Song, L. Li, C. Lei*, Z. Nie. Modular combination of proteolysis-responsive transcription and spherical nucleic acids for smartphone-based colorimetric detection of protease biomarkers. Anal. Chem., 2021, 93, 3517–3525. J. Zhao#, Z. Tan#, L. Wang*, C. Lei*, Z. Nie. A ligation-driven CRISPR-Cas biosensing platform for non-nucleic acid target detections. Chem. Commun., 2021, 57, 7051–7054 . K. Shi, L. Cao, F. Liu, S. Xie, S. Wang, Y. Huang, C. Lei*, Z. Nie. Amplified and label-free electrochemical detection of a protease biomarker by integrating proteolysis-triggered transcription. Biosens. Bioelectron., 2021, 190, 113372. M. Yang#, K. Shi#, F. Liu, W. Kang, C. Lei*, Z. Nie*. Coupling of proteolysis-triggered transcription and CRISPR-Cas12a for ultrasensitive protease detection. Sci. China Chem., 2021, 64, 330–336. X. Luo, J. Zhao, X. Xie, F. Liu, P. Zeng, C. Lei*, Z. Nie. Proteolysis-responsive rolling circle transcription assay enabling femtomolar sensitivity detection of a target protease biomarker. Anal. Chem., 2020, 92, 16314–16321. F. Liu, M. Yang, W. Song, X. Luo, R. Tang, Z. Duan, W. Kang, S. Xie, Q. Liu, C. Lei*, Y. Huang, Z. Nie*, S. Yao. Target-activated transcription for the amplified sensing of protease biomarkers. Chem. Sci., 2020, 11, 2993–2998. S. Wang, P. Zeng, X. Zhu, C. Lei*, Y. Huang, Z. Nie*. Chimeric peptides self-assembling on titanium carbide MXenes as biosensing interfaces for activity assay of post-translational modification enzymes. Anal. Chem., 2020, 92, 8819–8826. G. Feng#, X. Luo#, X. Lu, S. Xie, L. Deng, W. Kang, F. He, J. Zhang, C. Lei*, B, Lin, Y. Huang, Z. Nie*, S. Yao. Engineering of nucleic acids and synthetic cofactors as holo sensors for probing signaling molecules in the cellular membrane microenvironment. Angew. Chem. Int. Ed., 2019, 58, 6590−6594. S. Wang, W. Song, S. Wei, S. Zeng, S. Yang, C. Lei*, Y. Huang, Z. Nie*, S. Yao. Functional titanium carbide MXenes-loaded entropy-driven RNA explorer for long noncoding RNA PCA3 imaging in live cells. Anal. Chem., 2019, 91, 8622−8629. S. Wang, S. Wei, S. Wang, X. Zhu, C. Lei*, Y. Huang, Z. Nie*, S. Yao. Chimeric DNA-functionalized titanium carbide MXenes for simultaneous mapping of dual cancer biomarkers in living cells. Anal. Chem., 2019, 91, 1651−1658. X. Zhu, L. Fan, S. Wang, C. Lei*, Y. Huang, Z. Nie*, S. Yao. Phospholipid-tailored titanium carbide nanosheets as a novel fluorescent nanoprobe for activity assay and imaging of phospholipase D. Anal. Chem., 2018, 90, 6742−6748. C. Lei, H. Dai, Y. Fu*, Y. Ying, Y. Li*. Colorimetric sensor array for thiols discrimination based on urease-metal ion pairs. Anal. Chem., 2016, 88, 8542−8547. C. Lei, Z. Wang, Z. Nie, H. Deng, H. Hu, Y. Huang*, S. Yao. Resurfaced fluorescent protein as a sensing platform for label-free detection of copper(II) ion and acetylcholinesterase activity. Anal. Chem., 2015, 87, 1974−1980. C. Lei, Y. Huang, Z. Nie*, J. Hu, L. Li, G. Lu, Y. Han, S. Yao. Supercharged fluorescent protein as a versatile probe for homogenous DNA detection and methylation analysis. Angew. Chem. Int. Ed., 2014, 53, 8358–8362.