化学生物传感全国重点实验室/生物纳米与分子工程湖南省重点实验室/湖南大学化学化工学院教授，博士生导师，分析化学学科负责人，教育部青年长江学者，湖南省杰出青年基金获得者，湖南大学岳麓学者，主要研究兴趣是利用DNA分子进行材料构建、信息编码、逻辑计算、信号放大，以及生物医学方面的应用研究。先后主持和参与国家和省部级科研项目近20项，共发表研究论文200多篇，总引用次数超过10000次（H因子55），获发明专利10项。 教育背景 2009-2011：美国佛罗里达大学 联合培养博士 2005-2011：湖南大学化学化工学院 理学博士 2001-2005：湖南大学化学化工学院 理学学士 工作履历 2019-至今：湖南大学化学化工学院 教授 2015-2019：湖南大学化学化工学院 副教授 2012-2015：湖南大学化学化工学院 助理教授 2011-2017：湖南大学化学化工学院 博士后

利用DNA分子进行信息编码、逻辑计算、信号放大，以及在生物医学方面的应用研究

J. Li*, R. Chang, Y. Chen, W. Zhang, Y. Lan, D. Qiu, K. Wang, J. Huang*. Spatiotemporally controllable DNA nanomachines for fluorescent bioimaging. CCS Chemistry, 2025, accepted. Y. Chen, Z. Guo, J. Li, K. Wang, J. Huang*. Smart nucleic acid nanodrug delivery system for precision therapeutics. Coordination Chemistry Reviews, 2025, 535, 216673. Y. Chen, Y. Guo, W. Chen, J. Fan, J. Li, J. Song, X. Yang, K. Wang, J. Huang*. Sequentially activated smart DNA nanospheres for photoimmunotherapy and immune checkpoint blockade. Advanced Science, 2025, 12, 2410632. J. Li*, Q. Jiang, D. Qiu, M. Chen, W. Zhang, Y. Li, J. Huang*, Q. Xu*. Programming an amplified and self-calibrated electrochemical biosensor with high sensitivity, reproducibility, and stability for ORAOV 1 detection. Analytical Chemistry, 2025, 97, 3449-3459. J. Li*, W. Zhang, R. Chang, Y. Lan, D. Qiu, K. Wang, J. Huang*, Q. Xu*. A magnetic separation-assisted auto-cyclic primer extension for OSCC-associated salivary miRNA detection. Biosensors and Bioelectronics, 2025, 269, 116936. D. Qiu, J. Li*, Q. Jiang, W. Zhang, Y. Lan, R. Chang, C. Ma, J. Huang*, Q. Xu*. Entropy-driven “two-way signal output” cyclic circuit: an ultra-sensitive electrochemical biosensor for non-invasive ORAOV 1 detection. Talanta, 2025, 286, 127535. Y. Lang, R. Chang, W. Zhang, D. Qiu, C. Liang, J. Huang*, Q. Xu*, J. Li*. Proximity-induced DNA Ag NC enhancement with a DNA-fueled molecule machine for lung cancer-associated miRNA detection. Chemical Communications, 2025, 61, 4995-4998. Y. Chen, X. Liu, Y. Guo, S. Liu, J. Wang, X. He, X. Yang, K. Wang, J. Huang*. Tiny pH-resolved DNA nanospheres for cellular pyroptosis or apoptosis regulation. Nano Letters, 2024, 24, 13075-13084. S. Liu, J. Wang, Y. Chen, J. Fan, B. Du, R. Liu, X. Zhu, K. Wang, N. Xie*, J. Huang*. Modular assembled localized hybridization chain reaction for in situ mRNA amplified imaging. Nano Letters, 2024, 24, 11590-11598. Y. Chen, Y. Guo, J. Wang, R. Liu, X. Yang, K. Wang, Y. Pu*, H. Shi*, J. Huang*. Engineering self-catabolic DNAzyme nanospheres for synergistic anticancer therapy. Science China Chemistry, 2024, 67, 2412-2422. J. Li*, M. Chen, Q. Jiang, W. Zhang, Y. Lan, M. M. Ahmed, C. Ma, J. Huang*, Q. Xu*. Upgraded and light-up biosensing platform: entropy-driven catalysis circuit manipulates the configuration transformation of novel DNA silver nanoclusters on the graphene oxide surface. Analytical Chemistry, 2024, 96, 9209-9217. J. Li*, M. Chen, Q. Jiang, W. Zhang, S. Wang, J. Huang*, Q. Xu*. A highly cost-effective and sensitive biosensing platform: fuel-propelled DNA nanomachines modulate fluorescence switching of DNA. Microchemical Journal, 2024, 203, 110880. J. Li*, Q. Jiang, M. Chen, W. Zhang, R. Liu, J. Huang*, Q. Xu*. An attomolar-level electrochemical DNA biosensor based on target-triggered and entropy-driven catalytic ampliffcation integrated with AuNPs@ZIF-8 nanocomposites for oral cancer overexpressed 1 detection. Analytica Chimica Acta, 2024, 1287, 342055. J. Li*, X. Liu, J. Wang, Q. Jiang, M. Chen, W. Zhang, Y. Chen, Y. Pu*, J. Huang*. Self-assembled DNA nanospheres: design and applications. Chemistry, 2023, 5, 1882-1910. Z. Liu, N. Xie, J. Li, X. Fu, K. Wang, H. Shi*, J. Huang*. Self-priming DNA polymerization-propelled stochastic walkers on magnetic microbeads for amplified detection of miRNA. Analytical Chemistry, 2023, 95, 4529-4535. J. Wang, X. Fu, S. Liu, R. Liu, J. Li, K. Wang, J. Huang*. Catalyst-accelerated circular cascaded DNA circuits: simpler design, faster speed, higher gain. Small, 2023, 2205903. J. Wang, J. Li, Y. Chen, R. Liu, Y. Wu, J. Liu, X. Yang, K. Wang, J. Huang*. Size-controllable and self-assembled DNA nanosphere for amplified microRNA imaging through ATP-fueled cyclic dissociation. Nano Letters, 2022, 22, 8216-8223. J. Li, S. Liu, J. Wang, R. Liu, X. Yang, K. Wang, J. Huang*. Photocaged amplified FRET nanoflares: spatiotemporal controllable of mRNA-powered nanomachines for precise and sensitive microRNA imaging in live cells. Nucleic Acids Research, 2022, 50, e40. J. Xu, R. Jiang, Y. Feng, Z. Liu, J. Huang*, C. Ma*, K. Wang. Functional nucleic acid-based fluorescent probes for metal ion detection. Coordination Chemistry Reviews, 2022, 459, 214453. Y. Yang, T. Luo, Y. He, Z. Deng, J. Li, H. Liu, J. Nie, D. Wang, J. Huang*, S. Zhong*. Nanoflare couple：multiplexed mRNA imaging and logic-controlled combinational therapy. Analytical Chemistry, 2022, 94, 12204-12212. N. Xie, H. Wang, J. Li, K. Wang, J. Huang*. Dual amplification of bio-barcode and auto-cycling primer extention for highly sensitive detection of miRNA. Chemical Communications, 2022, 58, 10221-10224. S. Liu, Z. Liu, H. Wang, J. Wang, R. Liu, K. Wang, J. Huang*. A novel FRET-based dendritic hybridization chain reaction for tumor-related mRNA imaging. Chemical Communications, 2022, 58, 1414-1417. H. Wang, J. Wang, R. Liu, Z. Liu, K. Wang, J. Huang*. Auto-cycling primer extension for amplified microRNA detection. Chemical Communications, 2022, 58, 6020-6023. J. Li, J. Huang*. Fuel-powered DNA nanomachines for biosensing and cancer therapy. ChemPlusChem, 2022, 87, e202200098. Y. Ouyang, Y. Liu, Y. Deng, H. He, J. Huang*, C. Ma*, K. Wang. Recent advances in biosensor for DNA glycosylase activity detection. Talanta, 2022, 239, 123144. K. Quan, J. Wang, J. Li, K. Wang, J. Huang*. An endogenous miRNA-initiated hybridization chain reaction and subsequent DNAzyme activation for cellular theranostics. CCS Chemistry, 2021, 3, 3548-3558. L. Lan#, J. Huang#, M. Liu, Y. Yin, C. Wei, Q. Cai*, X. Meng*. Polymerization and isomerization cyclic amplification for nucleic acid detection with attomolar sensitivity. Chemical Science, 2021, 12, 4509-4518. J. Li, A. Wang, X. Yang, K. Wang, J. Huang*. Orderly-assembled, self-powered FRET flares for microRNA imaging in live cells. Analytical Chemistry, 2021, 93, 6270-6277. Q. Lin, S. Cai, B. Zhou, K. Wang, L. Jian*, J. Huang*. Dual-microRNA-regulation of singlet oxygen generation by DNA-tetrahedron-based molecular logic device. Chemical Communications, 2021, 57, 3873-3876. S. Cai, J. Wang, J. Li, B. Zhou, C. He, X. Meng*, J. Huang*, K. Wang. A self-assembled DNA nanostructure as a FRET nanoflare for intracellular ATP imaging. Chemical Communications, 2021, 57, 6257-6260. Y. Liu, Z. Liu, R. Liu, K. Wang, H. Shi*, J. Huang*. A MnO2 nanosheet-mediated photo-controlled DNAzyme for intracellular miRNA cleavage to suppress cell growth. Analyst, 2021, 146, 3391-3398. Y. Yang, Y. He, Z. Deng, J. Li, X. Li, J. Huang*, S. Zhong*. An autonomous self-cleavage DNAzyme walker for live cell microRNA imaging. ACS Applied Bio Materials, 2020, 3, 6310-6318. Y. Yang, Y. He, Z. Deng, J. Li, J. Huang*, S. Zhong*. Intelligent nanoprobe: acid-responsive drug release and in situ evaluation of its own therapeutic effect. Analytical Chemistry, 2020, 92, 12371-12378. J. Li, J.Wang, S. Liu, N. Xie, K. Quan, Y. Yang, X. Yang, J. Huang*, K.Wang*. Amplified FRET nanoflare: An endogenous mRNA-powered nanomachine for intracellular microRNA imaging. Angewandte Chemie International Edition, 2020,59,20104-20111. N. Xie, H. Wang, K. Quan, F. Feng*, J. Huang*, K. Wang*. Self-assembled DNA-based geometric polyhedrons: construction and applications. Trends in Analytical Chemistry, 2020, 126, 115844. K. Quan, C. Yi, X. Yang, X. He, J. Huang*, K. Wang*. FRET-based nucleic acid probes: basic designs and applications in bioimaging. Trends in Analytical Chemistry, 2020, 124, 115784. J. Wang, J. Li, S. Liu, X. Meng, X. Yang, J. Huang*, K. Wang*. An amplified AND logic platform for cell identification. Chemical Communications, 2020, 56, 11267-11270. Y. Wu, J. Li, K. Quan, X. Meng, Q. Guo, X. Yang, J. Huang*, K. Wang*. DNAzyme cascade for amplified detection of intracellular miRNA. Chemical Communications, 2020, 56, 10163-10166. A. Wang, Q. Lin, S. Liu, J. Li, J. Wang, K. Quan, X. Yang, J. Huang*, K. Wang. Aptamer-tethered self-assembled FRET-flares for micrRNA imaging in living cancer cells. Chemical Communications, 2020, 56, 2463-2466. Q. Lin, A.Wang, S. Liu, J. Li, J. Wang, K. Quan, X. Yang, J. Huang*, K. Wang. A DNA-tetrahedron-based molecular computation device for logic sensing of dual microRNAs in living cells. Chemical Communications, 2020, 56, 5303-5306. J. Li, S. Cai, B. Zhou, X. Meng, Q. Guo, X. Yang, J. Huang*, K. Wang*. Photocaged FRET nanoflares for intracelluar microRNA imaging. Chemical Communications, 2020, 56, 6126-6129. J. Li, K. Quan, Y. Yang, X. Yang, X. Meng, J. Huang*, K. Wang*. Engineering DNAzyme cascade for signal transduction and amplification. Analyst, 2020, 145, 1925-1932.